PMID- 876029
OWN - NLM
STAT- MEDLINE
DCOM- 19770812
LR  - 20190725
IS  - 0026-8925 (Print)
IS  - 0026-8925 (Linking)
VI  - 152
IP  - 3
DP  - 1977 Apr 29
TI  - Extrachromosomal inheritance in Schizosaccharomyces pombe. III. Isolation and 
      characterization of paromomycin-resistant mutants.
PG  - 319-24
AB  - In the antimycin--resistant mutant anar-8 of the fission yeast 
      Schizosaccharomyces pombe (Sch.p.) spontaneous mutants were isolated showing high 
      resistance to the aminoglycoside antibiotic paromomycin. All mutants were 
      resistant to the structurally related antibiotic neomycin. Tetrad analysis, 
      mitotic segregation analysis, and mitotic haploidization revealed 
      extrachromosomal, very likely mitochondrial inheritance. In contrast to the rapid 
      segregation of mitochondrial markers in zygotic clones of Saccharomyces 
      cerevisiae (S.c.) the heteroplasmic state of diploids proved to persist for at 
      least 50 generations after zygote formation. Stationary cultures of the 
      paromomycin-resistant mutants parr-106 and parr-112 contain up to 6% 
      respiratory-deficient mutants, but no reversion to paromomycin-sensitivity was 
      observed among 1700-1800 colonies tested. The ability of mutant anar-8 to produce 
      spontaneously respiratory-deficient mutants could be separated from the 
      antimycin-resistant phenotype of anar-8.
FAU - Del Giudice, L
AU  - Del Giudice L
FAU - Wolf, K
AU  - Wolf K
FAU - Seitz, G
AU  - Seitz G
FAU - Burger, G
AU  - Burger G
FAU - Lang, B
AU  - Lang B
FAU - Kaudewitz, F
AU  - Kaudewitz F
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Mol Gen Genet
JT  - Molecular & general genetics : MGG
JID - 0125036
RN  - 61JJC8N5ZK (Paromomycin)
SB  - IM
MH  - Ascomycota/*drug effects
MH  - Drug Resistance, Microbial
MH  - *Extrachromosomal Inheritance
MH  - *Mutation
MH  - Paromomycin/*pharmacology
MH  - Schizosaccharomyces/*drug effects/isolation & purification
EDAT- 1977/04/29 00:00
MHDA- 1977/04/29 00:01
CRDT- 1977/04/29 00:00
PHST- 1977/04/29 00:00 [pubmed]
PHST- 1977/04/29 00:01 [medline]
PHST- 1977/04/29 00:00 [entrez]
AID - 10.1007/BF00693086 [doi]
PST - ppublish
SO  - Mol Gen Genet. 1977 Apr 29;152(3):319-24. doi: 10.1007/BF00693086.