PMID- 8761025
OWN - NLM
STAT- MEDLINE
DCOM- 19960920
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 59
IP  - 9
DP  - 1996
TI  - Studies on the immunoglobulin-E system of the common marmoset in comparison with 
      human data.
PG  - 719-30
AB  - In the common marmoset (Callithrix jacchus jacchus) immunoglobulin E (IgE) serum 
      levels and IgE synthesis of peripheral blood mononuclear cells (PBMC) in vitro 
      were investigated in order to look for homologies to the human system. While IgE 
      was not found in marmoset blood plasma with three commercial antihuman IgE-kits 
      with monoclonal antibodies (mAbs), two other kits using polyclonal antibodies 
      against human IgE revealed detectable IgE concentrations of up to 10 kU/liter in 
      plasma samples of 19 out of 21 marmosets. In accord with human data, rhIL-4 
      showed biological functions under in vitro conditions in PBMC of the New World 
      monkey. Proliferation, measured by 3H-thymidine incorporation, of isolated PBMC 
      of marmosets could be induced by rhIL-4. FACScan analysis showed an enhanced 
      expression of the low affinity IgE receptor CD23 (Fc epsilon RII) on CD20+ B 
      lymphocytes after incubation with rhIL-4. Furthermore, PBMC from marmosets could 
      be stimulated by IL-4 alone or in combination with dexamethasone as well as with 
      lipopolysaccharide (E. coli) to produce IgE in culture. The results indicate that 
      Callithrix jacchus is using an IgE system that is rather similar to that of 
      humans, although not completely identical. Antihuman mAbs and rhIL-4 can be used 
      to investigate IgE regulation in vitro of marmoset PBMC. These data encourage the 
      development of a primate animal model for studying possible modifications of the 
      IgE system under pathological conditions to find new therapeutic strategies in 
      atopic diseases.
FAU - Schmidt, S
AU  - Schmidt S
AD  - University Medical Center, Free University Berlin, Institute of Toxicology and 
      Embryopharmacology, Germany.
FAU - Neubert, R
AU  - Neubert R
FAU - Schmitt, M
AU  - Schmitt M
FAU - Neubert, D
AU  - Neubert D
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Antibodies)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Receptors, IgE)
RN  - 0 (Recombinant Proteins)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Antibodies
MH  - Antibodies, Monoclonal
MH  - Antibody Formation/drug effects
MH  - B-Lymphocytes/drug effects/immunology
MH  - Callithrix/*immunology
MH  - Cell Division
MH  - Cells, Cultured
MH  - Dexamethasone/pharmacology
MH  - Escherichia coli
MH  - Flow Cytometry
MH  - Humans
MH  - Immunoglobulin E/biosynthesis/*blood
MH  - Interleukin-4/pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Lymphocyte Activation
MH  - Lymphocytes/drug effects/*immunology
MH  - Receptors, IgE/biosynthesis
MH  - Recombinant Proteins/pharmacology
MH  - Species Specificity
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 002432059600358X [pii]
AID - 10.1016/0024-3205(96)00358-x [doi]
PST - ppublish
SO  - Life Sci. 1996;59(9):719-30. doi: 10.1016/0024-3205(96)00358-x.