PMID- 8761307
OWN - NLM
STAT- MEDLINE
DCOM- 19961010
LR  - 20091119
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 13
IP  - 4
DP  - 1996 Aug 15
TI  - Autocrine hepatocyte growth factor/scatter factor-Met signaling induces 
      transformation and the invasive/metastastic phenotype in C127 cells.
PG  - 853-6
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is a pleiotropic effector of 
      cells expressing the Met tyrosine kinase receptor. C127 is a non-tumorigenic 
      mouse cell line which expresses negligible levels of HGF/SF and Met proteins. In 
      the present report we have generated C127 cells which overexpress HGF/SF and/or 
      Met proteins, and have analysed the effect of HGF/SF-Met signaling in these 
      cells. We show that this signaling pathway stimulates the growth and invasiveness 
      of C127 cells in vitro and that cells overexpressing both HGF/SF and Met proteins 
      (but neither alone) are phenotypically transformed and highly tumorigenic and 
      metastatic in vivo. Our data unequivocally demonstrates the autocrine dependency 
      of HGF/SF-Met-induced transformation and metastasis in this system and supports 
      the theory that the inappropriate expression of HGF/SF and Met proteins could 
      play a role in the development and spread of human tumors. In addition, this 
      system may be useful for identifying metastasis-associated genes that are 
      activated by HGF/SF-Met signaling.
FAU - Jeffers, M
AU  - Jeffers M
AD  - ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, 
      Frederick, Maryland 21702, USA.
FAU - Rong, S
AU  - Rong S
FAU - Anver, M
AU  - Anver M
FAU - Vande Woude, G F
AU  - Vande Woude GF
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Transformation, Neoplastic/genetics
MH  - Female
MH  - Hepatocyte Growth Factor/*metabolism
MH  - Humans
MH  - Mice
MH  - Mice, Nude
MH  - Neoplasm Invasiveness/genetics
MH  - Neoplasm Metastasis/genetics
MH  - Phenotype
MH  - Proto-Oncogene Proteins c-met
MH  - Receptor Protein-Tyrosine Kinases/*metabolism
MH  - *Signal Transduction
EDAT- 1996/08/15 00:00
MHDA- 1996/08/15 00:01
CRDT- 1996/08/15 00:00
PHST- 1996/08/15 00:00 [pubmed]
PHST- 1996/08/15 00:01 [medline]
PHST- 1996/08/15 00:00 [entrez]
PST - ppublish
SO  - Oncogene. 1996 Aug 15;13(4):853-6.