PMID- 8761441
OWN - NLM
STAT- MEDLINE
DCOM- 19961001
LR  - 20190512
IS  - 0143-3334 (Print)
IS  - 0143-3334 (Linking)
VI  - 17
IP  - 8
DP  - 1996 Aug
TI  - Metabolic activation and DNA binding of food mutagens and other environmental 
      carcinogens in human mammary epithelial cells.
PG  - 1769-72
AB  - Cultures of human mammary epithelial cells were treated with one of seven 
      heterocyclic amine food mutagens [2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 
      2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ), 
      2-amino-3,8-di-methylimidazo[4,5-f]quinoxaline (MeIQx), 
      2-amino-3,4,8-trimethylimidazo[4,5-f]quinoxaline (4,8-DiMeIQx), 
      2-amino-3,7,8-trimethylimidazo[4,5-f]quinoxaline (7,8-DiMeIQx), 
      2-amino-3,4,7,8-tetramethylimidazo[4,5-f]quinoxaline (4,7,8-TriMeIQx) or 
      2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine (PhIP)], four nitropyrenes 
      (1-nitropyrene (1-NP), 1,3-dinitropyrene (1,3-DNP), 1,6-dinitropyrene (1,6-DNP) 
      or 1,8-dinitropyrene (1,8-DNP)] or the polycyclic aromatic hydrocarbon 
      dibenzo[a,l]pyrene (DB[a,l]P). DNA isolated from the cultures was analysed by 
      32P-post-labelling and in each case the presence of carcinogen-DNA adducts was 
      detected. The patterns and numbers of adducts obtained when human mammary cell 
      DNA digests were separated on polyethyleneimine-cellulose TLC were found to 
      closely resemble those previously demonstrated to be present in the DNA of 
      tissues from rodents and other primates treated with the same agents. Up to six 
      DNA adducts were detected in human breast cells treated with IQ and MeIQ. Fewer 
      adducts (1-3) were detected following treatment with MeIQx or its methylated 
      derivatives, whilst PhIP gave rise to at least four distinct adduct spots. Five 
      adduct spots were detected in breast cells treated with DB[a,l]P or with 1-NP, 
      but fewer adduct spots were formed by 1,3-, 1,6- and 1,8-DNP. These data 
      demonstrate the ability of human breast epithelial cells to activate to DNA 
      binding species a range of carcinogenic compounds known to be present in the 
      human diet or to which humans are known to be exposed environmentally.
FAU - Carmichael, P L
AU  - Carmichael PL
AD  - Institute of Cancer Research, Haddow Laboratories, Belmont, Sutton, Surrey, UK.
FAU - Stone, E M
AU  - Stone EM
FAU - Grover, P L
AU  - Grover PL
FAU - Gusterson, B A
AU  - Gusterson BA
FAU - Phillips, D H
AU  - Phillips DH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - 0 (Carcinogens, Environmental)
RN  - 0 (DNA Adducts)
RN  - 0 (Mutagens)
RN  - 0 (Phosphorus Radioisotopes)
SB  - IM
MH  - Biotransformation
MH  - Breast/*drug effects/metabolism
MH  - Carcinogens, Environmental/*pharmacokinetics
MH  - Cells, Cultured
MH  - DNA Adducts/analysis
MH  - Epithelium/drug effects/metabolism
MH  - Female
MH  - *Food
MH  - Humans
MH  - Mutagens/*pharmacokinetics
MH  - Phosphorus Radioisotopes
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
AID - 10.1093/carcin/17.8.1769 [doi]
PST - ppublish
SO  - Carcinogenesis. 1996 Aug;17(8):1769-72. doi: 10.1093/carcin/17.8.1769.