PMID- 8762180
OWN - NLM
STAT- MEDLINE
DCOM- 19961031
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 210
IP  - 1
DP  - 1996 May 24
TI  - Increased beta-amyloid precursor protein mRNA in the rat cerebral cortex and 
      hippocampus after chronic systemic atropine treatment.
PG  - 13-6
AB  - Rats were treated with once-daily subcutaneous injections of atropine or normal 
      saline for 10 days. Cryostat sections of fresh-frozen brain were subjected to 
      quantitative muscarinic receptor ([3H]quinuclidinylbenzilate (QNB)) binding 
      autoradiography, and quantitative in-situ hybridization autoradiography for 
      beta-amyloid precursor protein (beta-APP) mRNA using an oligonucleotide probe 
      recognizing all major isoforms. QNB binding in the atropine-treated group was 
      increased 6-7% in the areas measured (dentate gyrus, CA1, and cerebral cortex), 
      confirming that the treatment was effective in inducing muscarinic receptor 
      upregulation. Hybridization signal for beta-APP mRNA was increased 15-20% in the 
      atropine-treated group in the same regions. As chronic atropine treatment models 
      the muscarinic effects of cholinergic denervation, these results suggest that 
      age-related cholinergic neuron loss may result in upregulation of beta-APP.
FAU - Beach, T G
AU  - Beach TG
AD  - University of British Columbia, Vacouver Hospital and Health Sciences Centre, 
      Department of Pathology, Canada.
FAU - Walker, D G
AU  - Walker DG
FAU - Cynader, M S
AU  - Cynader MS
FAU - Hughes, L H
AU  - Hughes LH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Muscarinic Antagonists)
RN  - 0 (RNA, Messenger)
RN  - 6581-06-2 (Quinuclidinyl Benzilate)
RN  - 7C0697DR9I (Atropine)
SB  - IM
MH  - Amyloid beta-Protein Precursor/*genetics
MH  - Animals
MH  - Atropine/*pharmacology
MH  - Autoradiography
MH  - Cerebral Cortex/*drug effects
MH  - Gene Expression/drug effects
MH  - Hippocampus/*drug effects
MH  - In Situ Hybridization
MH  - Muscarinic Antagonists/*pharmacology
MH  - Quinuclidinyl Benzilate/pharmacology
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Time Factors
EDAT- 1996/05/24 00:00
MHDA- 1996/05/24 00:01
CRDT- 1996/05/24 00:00
PHST- 1996/05/24 00:00 [pubmed]
PHST- 1996/05/24 00:01 [medline]
PHST- 1996/05/24 00:00 [entrez]
AID - 0304394096126501 [pii]
AID - 10.1016/0304-3940(96)12650-1 [doi]
PST - ppublish
SO  - Neurosci Lett. 1996 May 24;210(1):13-6. doi: 10.1016/0304-3940(96)12650-1.