PMID- 8764020
OWN - NLM
STAT- MEDLINE
DCOM- 19961107
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 70
IP  - 8
DP  - 1996 Aug
TI  - Species-specific effects of the hcf-1 gene on baculovirus virulence.
PG  - 5123-30
AB  - The host cell-specific factor 1 gene (hcf-1) of the baculovirus Autographa 
      californica nuclear polyhedrosis virus (AcMNPV) is required for the efficient 
      replication and/or stability of reporter plasmids carrying an AcMNPV-derived 
      origin of DNA replication in a cell-specific manner; hcf-1 is required for 
      reporter plasmid replication or stability in TN-368 cells, a cell line derived 
      from the cabbage looper Trichoplusia ni, but not in IPLB-SF-21 (SF-21) cells, a 
      cell line derived from the fall armyworm Spodoptera frugiperda (A. Lu and L. K. 
      Miller, J. Virol. 69:6265-6272, 1995). To further define the function of hcf-1, 
      recombinant viruses with null mutations in hcf-1 were constructed in SF-21 cells 
      and the phenotype of the mutants was determined in selected cell lines as well as 
      in insect larvae. In S.frugiperda larvae and SF-21 cells, the phenotype of hcf-1 
      mutants was indistinguishable from that of wild-type AcMNPV. In T. ni larvae as 
      well as T. ni-derived cell lines, hcf-1 mutants exhibited a mutant phenotype. In 
      TN-368 cells, the replication of hcf-1 mutants was extremely impaired; the 
      phenotype included a defect in viral DNA replication, late gene transcription, 
      and virus production as well as a complete cessation of host and viral protein 
      synthesis. In another cell line derived from T. ni, the BTI-TN5B1-4 cell line, 
      the hcf-1 mutants exhibited a less severe phenotype. In T. ni larvae, the 
      infectivity of the budded form of hcf-1 mutants was decreased significantly 
      (50-fold), although no difference in the oral infectivity of the occluded form 
      was observed. T. ni larvae infected with hcf-1 mutants by either oral or 
      hemocoelic routes, however, died 20 to 30% more slowly than those infected with 
      wild-type AcMNPV. These data indicate that there is a host-specific requirement 
      for hcf-1 and that it exerts cell line-specific effects and possibly 
      tissue-specific effects on the rate at which the virus replicates, thereby 
      affecting the virulence of the virus in a species-specific manner.
FAU - Lu, A
AU  - Lu A
AD  - Department of Entomology, The University of Georgia, Athens 30602, USA.
FAU - Miller, L K
AU  - Miller LK
LA  - eng
GR  - AI 23719/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
SB  - IM
MH  - Animals
MH  - Baculoviridae/genetics/*pathogenicity
MH  - Gene Deletion
MH  - Genes, Viral/*genetics
MH  - Species Specificity
MH  - Spodoptera/*virology
MH  - Virulence/genetics
MH  - Virus Replication/genetics
PMC - PMC190467
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
PMCR- 1996/08/01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
PHST- 1996/08/01 00:00 [pmc-release]
AID - 10.1128/JVI.70.8.5123-5130.1996 [doi]
PST - ppublish
SO  - J Virol. 1996 Aug;70(8):5123-30. doi: 10.1128/JVI.70.8.5123-5130.1996.