PMID- 8764577
OWN - NLM
STAT- MEDLINE
DCOM- 19960919
LR  - 20191210
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 67
IP  - 2
DP  - 1996 Aug
TI  - Role of Ca2+ in differentiation mediated by nerve growth factor and dibutyryl 
      cyclic AMP in PC12 cells.
PG  - 530-9
AB  - Nerve growth factor (NGF) and dibutyryl cyclic AMP (dbcAMP) have synergistic 
      effects on the neurite outgrowth of rat pheochromocytoma PC12 cells. The sites of 
      interaction between NGF and dbcAMP have been studied extensively; however, the 
      role of Ca2+ in differentiation induced by the two agents remains unclear. To 
      understand whether intracellular Ca2+ is involved in the differentiation induced 
      by the two agents, PC12 cells were treated with NGF, dbcAMP, or NGF plus dbcAMP 
      for 2 days, and then effects on neurite outgrowth, ATP-induced Ca2+ influx, and 
      Ca2+ mobilization from intracellular Ca2+ pools were examined. NGF or dbcAMP 
      alone enhanced neurite outgrowth and Ca2+ accumulation by nonmitochondrial Ca2+ 
      pools or the thapsigargin (TG)-sensitive Ca2+ pool. The dbcAMP acted 
      synergistically with NGF to increase neurite outgrowth and to enlarge the 
      TG-sensitive Ca2+ pool. The synergistic effect occurred within the first hour of 
      treatment with dbcAMP plus NGF. On the other hand, dbcAMP abolished NGF's ability 
      to enhance ATP-induced influx of extracellular Ca2+ . Therefore, NGF and dbcAMP 
      induced different effects on Ca2+ signaling pathways through two different but 
      interacting pathways. In PC12 cells pretreated with TG to deplete the 
      TG-sensitive Ca2+ pool, the dbcAMP- or dbcAMP plus NGF-mediated neurite outgrowth 
      was significantly inhibited, whereas NGF-mediated neurite outgrowth was not 
      affected by TG pretreatment. Our results suggest that the intracellular 
      nonmitochondrial Ca2+ pools were changed in the differentiation process and were 
      necessary for the synergistic effect of NGF and dbcAMP.
FAU - Huang, C M
AU  - Huang CM
AD  - Institute of Biomedical Sciences, Academia Sinica, National Taiwan University, 
      Taipei, R.O.C.
FAU - Tsay, K E
AU  - Tsay KE
FAU - Kao, L S
AU  - Kao LS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Terpenes)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - 67526-95-8 (Thapsigargin)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adenosine Triphosphate/pharmacology
MH  - Animals
MH  - Bucladesine/*administration & dosage
MH  - Calcium/*physiology
MH  - Calcium-Transporting ATPases/antagonists & inhibitors
MH  - Cell Compartmentation/drug effects
MH  - Cell Differentiation/drug effects
MH  - Drug Synergism
MH  - Enzyme Inhibitors/pharmacology
MH  - Nerve Growth Factors/*administration & dosage
MH  - Neurons/cytology
MH  - PC12 Cells/*cytology
MH  - Rats
MH  - Signal Transduction
MH  - Terpenes/pharmacology
MH  - Thapsigargin
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
AID - 10.1046/j.1471-4159.1996.67020530.x [doi]
PST - ppublish
SO  - J Neurochem. 1996 Aug;67(2):530-9. doi: 10.1046/j.1471-4159.1996.67020530.x.