PMID- 8766019
OWN - NLM
STAT- MEDLINE
DCOM- 19961017
LR  - 20190726
IS  - 0031-6768 (Print)
IS  - 0031-6768 (Linking)
VI  - 432
IP  - 3
DP  - 1996 Jul
TI  - Roles of tyrosine kinase in insulin action on cell volume of fetal rat type II 
      pneumocyte.
PG  - 571-3
AB  - The aim of the present study was to investigate the roles of tyrosine kinase (TK) 
      in the insulin action on cell volume in fetal rat (20-day gestational age) type 
      II pneumocyte. Insulin (100 nmol/l) increased cell volume, and this insulin (100 
      nmol/l) action was completely blocked by 50 micromol/l bumetanide (BMT) and 10 
      micromol/l amiloride (AML). This observation indicates that 100 nmol/l insulin 
      activates BMT-sensitive Na+/K+/2Cl- cotransporter and AML-sensitive pathways. The 
      stimulatory action of 100 nmol/l insulin on BMT-sensitive Na+/K+/2Cl- 
      cotransporter was completely abolished by 10 micromol/l lavendustin A (LAV-A, an 
      inhibitor of TK), however 100 nmol/l insulin could stimulate AML-sensitive 
      pathways even in LAV-A (10 micromol/l)-treated cells. These observations indicate 
      that the insulin (100 nmol/l) action on the BMT-sensitive Na+/K+/2Cl- 
      cotransporter is mediated through TK-dependent pathways, while 100 nmol/l insulin 
      requires a TK-independent pathway to show the stimulatory action on the 
      AML-sensitive pathways. From these observations we conclude that TK-dependent and 
      -independent pathways are involved in the insulin (100 nmol/l) signaling in fetal 
      rat type II pneumocyte.
FAU - Marunaka, Y
AU  - Marunaka Y
AD  - MRC Group in Lung Development, Hospital for Sick Children Research Institute, 
      University of Toronto, Faculty of Medicine, Toronto, Ontario, Canada M5G 1X8.
FAU - Shintani, Y
AU  - Shintani Y
FAU - Sugimoto, E
AU  - Sugimoto E
FAU - Niisato, N
AU  - Niisato N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Pflugers Arch
JT  - Pflugers Archiv : European journal of physiology
JID - 0154720
RN  - 0 (Carrier Proteins)
RN  - 0 (Diuretics)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Insulin)
RN  - 0 (Phenols)
RN  - 0 (Sodium Channels)
RN  - 0 (Sodium-Potassium-Chloride Symporters)
RN  - 0Y2S3XUQ5H (Bumetanide)
RN  - 3Y0G32G2RV (lavendustin A)
RN  - 7DZO8EB0Z3 (Amiloride)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
SB  - IM
MH  - Amiloride/pharmacology
MH  - Animals
MH  - Bumetanide/pharmacology
MH  - Carrier Proteins/metabolism
MH  - Cell Size/*drug effects
MH  - Cells, Cultured
MH  - Diuretics/pharmacology
MH  - Enzyme Inhibitors/pharmacology
MH  - Hypoglycemic Agents/*pharmacology
MH  - Insulin/*pharmacology
MH  - Lung/*cytology/drug effects/physiology
MH  - Phenols/pharmacology
MH  - Protein-Tyrosine Kinases/antagonists & inhibitors/*physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Sodium Channels/drug effects/metabolism
MH  - Sodium-Potassium-Chloride Symporters
EDAT- 1996/07/01 00:00
MHDA- 1996/07/01 00:01
CRDT- 1996/07/01 00:00
PHST- 1996/07/01 00:00 [pubmed]
PHST- 1996/07/01 00:01 [medline]
PHST- 1996/07/01 00:00 [entrez]
AID - 10.1007/s004240050171 [doi]
PST - ppublish
SO  - Pflugers Arch. 1996 Jul;432(3):571-3. doi: 10.1007/s004240050171.