PMID- 8766541
OWN - NLM
STAT- MEDLINE
DCOM- 19960916
LR  - 20131121
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 26
IP  - 7
DP  - 1996 Jul
TI  - Selective suppression of endothelial cell activation by arachidonic acid.
PG  - 1417-23
AB  - Endothelial cell (EC) activation plays a key role in inflammation, thrombosis and 
      organ rejection. Normally, EC are in a quiescent state in which their function is 
      to prevent coagulation and thrombosis, and to participate in the regulation of 
      leukocyte migration from the bloodstream into the tissue. Upon activation with 
      cytokines or other stimuli, EC up-regulate a number of genes, including 
      E-selectin (ELAM-1), intercellular adhesion molecule (ICAM)-1, vascular cell 
      adhesion molecule (VCAM)-1, interleukin (IL)-1, IL-8, tissue factor (TF), 
      plasminogen activator inhibitor-1 (PAI-1), MCP-1 (monocyte chemoattractant 
      protein-1) and endothelial cell inducible gene (ECI-6). Arachidonic acid (AA) is 
      produced by several cell types, including EC, and acts on various cells. We 
      report here that AA inhibits the up-regulation of some, but not all genes that 
      are induced with EC activation in a dose-dependent manner. AA suppresses 
      TNF-alpha, IL-1 alpha, LPS or PMA-induced E-selectin expression, as well as mRNA 
      accumulation of E-selectin, ICAM-1 and IL-8 stimulated by TNF-alpha. The 
      inhibition appears to be at the level of transcription. At the same time under 
      the same conditions AA does not, repress mRNA accumulation for PAI-1, ECI-6, 
      MCP-1 and VCAM-1. We suggest that the induced expression of AA with EC activation 
      may result in a negative feedback loop regulating further activation.
FAU - Stuhlmeier, K M
AU  - Stuhlmeier KM
AD  - New England Deaconess Hospital, Harvard Medical School, Boston, MA 02215, USA. 
      kstuhlme@nedhmail.nedh.harvard.edu
FAU - Tarn, C
AU  - Tarn C
FAU - Csizmadia, V
AU  - Csizmadia V
FAU - Bach, F H
AU  - Bach FH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (E-Selectin)
RN  - 0 (Immunosuppressive Agents)
RN  - 27YG812J1I (Arachidonic Acid)
SB  - IM
MH  - Animals
MH  - Aorta/cytology
MH  - Arachidonic Acid/metabolism/*pharmacology
MH  - E-Selectin/drug effects/genetics
MH  - Endothelium, Vascular/*drug effects/immunology/metabolism
MH  - Gene Expression Regulation/drug effects/immunology
MH  - Immunosuppressive Agents/*pharmacology
MH  - Swine
MH  - Transcription, Genetic/drug effects
MH  - Up-Regulation/drug effects/genetics/immunology
EDAT- 1996/07/01 00:00
MHDA- 1996/07/01 00:01
CRDT- 1996/07/01 00:00
PHST- 1996/07/01 00:00 [pubmed]
PHST- 1996/07/01 00:01 [medline]
PHST- 1996/07/01 00:00 [entrez]
AID - 10.1002/eji.1830260703 [doi]
PST - ppublish
SO  - Eur J Immunol. 1996 Jul;26(7):1417-23. doi: 10.1002/eji.1830260703.