PMID- 8766706
OWN - NLM
STAT- MEDLINE
DCOM- 19960923
LR  - 20191210
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 389
IP  - 3
DP  - 1996 Jul 8
TI  - 2-Nitrosofluorene and N-hydroxy-2-aminofluorene react with the 
      ubiquinone-reduction center (center N) of the mitochondrial cytochrome bc1 
      complex.
PG  - 233-7
AB  - We determined the sites of artificial electron transfer onto 2-nitrosofluorene 
      (NOF), a metabolite of carcinogenic 2- acetylaminofluorene in mitochondria and 
      isolated cytochrome bc1 complex. NOF-induced O2 consumption in mitochondria was 
      sensitive to antimycin A, but insensitive to myxothiazol. In the isolated 
      cytochrome bc1 complex, NOF induced rapid MOA-stilbene-insensitive reoxidation of 
      cytochrome b, whereas in the presence of antimycin A, reoxidation was very slow. 
      The corresponding hydroxylamine, N-hydroxy-2-aminofluorene (N-OH-AF), reduced 
      cytochrome b specifically through center N of the cytochrome bc1 complex. We 
      conclude that NOF and N-OH-AF bind to center N of the cytochrome bc1 complex and 
      act as electron acceptor and donor, respectively. The N-OH-AF/NOF interconversion 
      is considered to be involved in the cytotoxicity of 2-acetylaminofluorene in 
      vivo.
FAU - Klohn, P C
AU  - Klohn PC
AD  - Institut fur Toxikologie, Universitat Wurzburg, Germany.
FAU - Brandt, U
AU  - Brandt U
FAU - Neumann, H G
AU  - Neumann HG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Cytochrome b Group)
RN  - 0 (Fluorenes)
RN  - 0 (Methacrylates)
RN  - 0 (Nitroso Compounds)
RN  - 0 (Stilbenes)
RN  - 0 (Thiazoles)
RN  - 0 (Uncoupling Agents)
RN  - 103455-29-4 (3-methoxy-2-(2-styrylphenyl)propenic acid methyl ester)
RN  - 1339-63-5 (Ubiquinone)
RN  - 14844-07-6 (Dithionite)
RN  - 34E9V9B29K (2-nitrosofluorene)
RN  - 61M94X4V24 (N-hydroxy-2-aminofluorene)
RN  - 642-15-9 (Antimycin A)
RN  - 6VY98BQ7NB (myxothiazol)
RN  - EC 7.1.1.8 (Electron Transport Complex III)
RN  - MQD255M2ZO (Potassium Cyanide)
RN  - P4P3AC32ZB (Tetramethylphenylenediamine)
SB  - IM
MH  - Animals
MH  - Antimycin A/pharmacology
MH  - Cytochrome b Group/metabolism
MH  - Dithionite/pharmacology
MH  - Electron Transport Complex III/antagonists & inhibitors/*metabolism
MH  - Fluorenes/*metabolism/pharmacology
MH  - Male
MH  - Methacrylates
MH  - Mitochondria, Liver/drug effects/*metabolism
MH  - Nitroso Compounds/*metabolism/pharmacology
MH  - Oxidation-Reduction
MH  - Oxygen Consumption/drug effects
MH  - Potassium Cyanide/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Stilbenes/pharmacology
MH  - Tetramethylphenylenediamine/pharmacology
MH  - Thiazoles/pharmacology
MH  - Ubiquinone/*metabolism
MH  - Uncoupling Agents/pharmacology
EDAT- 1996/07/08 00:00
MHDA- 1996/07/08 00:01
CRDT- 1996/07/08 00:00
PHST- 1996/07/08 00:00 [pubmed]
PHST- 1996/07/08 00:01 [medline]
PHST- 1996/07/08 00:00 [entrez]
AID - 0014-5793(96)00592-3 [pii]
AID - 10.1016/0014-5793(96)00592-3 [doi]
PST - ppublish
SO  - FEBS Lett. 1996 Jul 8;389(3):233-7. doi: 10.1016/0014-5793(96)00592-3.