PMID- 8768787
OWN - NLM
STAT- MEDLINE
DCOM- 19961029
LR  - 20061115
IS  - 0748-6642 (Print)
IS  - 0748-6642 (Linking)
VI  - 27
IP  - 4
DP  - 1995
TI  - The short DNA sequences in the cytoplasm of Ehrlich ascites tumor cells are 
      tightly associated with proteins.
PG  - 321-8
AB  - Mouse Ehrlich tumor cells harbor extrachromosomal DNA elements in a 
      non-mitochondrial fraction of the cytoplasm (Abken et al., Proc. Natl. Acad. Sci 
      USA 90: 6518-6522, 1993). The cytoplasmic DNA sequences constitute a distinct 
      group of extrachromosomal genetic elements with common properties: (i) the DNA 
      molecules are 50-500 bp in length and of linear configuration, (ii) most of the 
      DNA sequences exhibit the potential to modulate the activity of a transcriptional 
      promoter, and (iii) the DNA elements are preferentially found in tumor cells, not 
      in cells with normal phenotype. Unexpectedly, the extrachromosomal DNA sequences 
      are found to be tightly associated with at least three proteins (52 kD, 62 kD, 64 
      kD) forming DNA-protein (DNP) complexes. The DNA-protein interaction is stable 
      during extraction with phenol and chloroform and during incubation with 
      proteinase K and pronase P, in the presence of detergents (SDS, NP40), 
      guanidine-HCl, high salt concentrations, and alkali. Hydrolysis of the DNA by 
      DNAse I makes the proteins of the DNP complex accessible to proteolytic 
      degradation. Western blot analyses imply that the proteins associated with the 
      cytoplasmic DNA sequences are antigenically related to nucleomatrix proteins that 
      are covalently bound to certain regions of chromosomal DNA. Covalent bonds 
      between the cytoplasmic DNA and the polypeptides would explain the unexpected 
      high stability of the cytoplasmic DNA-protein complexes in tumor cells.
FAU - Hegger, R
AU  - Hegger R
AD  - Klinik I fur Innere Medizin, Universitat zu Koln, Germany.
FAU - Abken, H
AU  - Abken H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Physiol Chem Phys Med NMR
JT  - Physiological chemistry and physics and medical NMR
JID - 8502230
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Neoplasm Proteins)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Carcinoma, Ehrlich Tumor/*metabolism/ultrastructure
MH  - Cell Nucleus/chemistry
MH  - Cytoplasm/*chemistry/metabolism
MH  - DNA, Neoplasm/*chemistry/metabolism
MH  - In Situ Hybridization
MH  - Mice
MH  - Neoplasm Proteins/chemistry/*metabolism
MH  - Phenotype
MH  - Tumor Cells, Cultured
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
PST - ppublish
SO  - Physiol Chem Phys Med NMR. 1995;27(4):321-8.