PMID- 8769419
OWN - NLM
STAT- MEDLINE
DCOM- 19961001
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 134
IP  - 4
DP  - 1996 Aug
TI  - Aberrant mitosis in fission yeast mutants defective in fatty acid synthetase and 
      acetyl CoA carboxylase.
PG  - 949-61
AB  - Two fission yeast temperature-sensitive mutants, cut6 and lsd1, show a defect in 
      nuclear division. The daughter nuclei differ dramatically in size (the phenotype 
      designated lsd, large and small daughter). Fluorescence in situ hybridization 
      (FISH) revealed that sister chromatids were separated in the lsd cells, but 
      appeared highly compact in one of the two daughter nuclei. EM showed asymmetric 
      nuclear elongation followed by unequal separation of nonchromosomal nuclear 
      structures in these mutant nuclei. The small nuclei lacked electron-dense nuclear 
      materials and contained highly compacted chromatin. The cut6+ and lsd1+ genes are 
      essential for viability and encode, respectively, acetyl CoA carboxylase and 
      fatty acid synthetase, the key enzymes for fatty acid synthesis. Gene disruption 
      of lsd1+ led to the lsd phenotype. Palmitate in medium fully suppressed the 
      phenotypes of lsd1. Cerulenin, an inhibitor for fatty acid synthesis, produced 
      the lsd phenotype in wild type. The drug caused cell inviability during mitosis 
      but not during the G2-arrest induced by the cdc25 mutation. A reduced level of 
      fatty acid thus led to impaired separation of non-chromosomal nuclear components. 
      We propose that fatty acid is directly or indirectly required for separating the 
      mother nucleus into two equal daughters.
FAU - Saitoh, S
AU  - Saitoh S
AD  - Department of Biophysics, Faculty of Science, Kyoto University, Japan.
FAU - Takahashi, K
AU  - Takahashi K
FAU - Nabeshima, K
AU  - Nabeshima K
FAU - Yamashita, Y
AU  - Yamashita Y
FAU - Nakaseko, Y
AU  - Nakaseko Y
FAU - Hirata, A
AU  - Hirata A
FAU - Yanagida, M
AU  - Yanagida M
LA  - eng
SI  - GENBANK/D83412
SI  - GENBANK/D83413
SI  - GENBANK/D83414
SI  - GENBANK/D83415
SI  - GENBANK/D83416
SI  - GENBANK/D83417
SI  - GENBANK/D83418
SI  - GENBANK/D83419
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Palmitates)
RN  - 17397-89-6 (Cerulenin)
RN  - EC 2.3.1.85 (Fatty Acid Synthases)
RN  - EC 6.4.1.2 (Acetyl-CoA Carboxylase)
SB  - IM
MH  - Acetyl-CoA Carboxylase/genetics/*metabolism
MH  - Amino Acid Sequence
MH  - Cell Division
MH  - Cell Nucleus/ultrastructure
MH  - Cerulenin/pharmacology
MH  - Chromosomes, Fungal
MH  - Cloning, Molecular
MH  - Enzyme Inhibitors/pharmacology
MH  - Fatty Acid Synthases/antagonists & inhibitors/genetics/*metabolism
MH  - Genes, Fungal/genetics
MH  - Mitosis/*physiology
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Palmitates/pharmacology
MH  - Phenotype
MH  - Schizosaccharomyces/*cytology/enzymology/genetics
MH  - Sequence Analysis, DNA
MH  - Temperature
PMC - PMC2120970
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
PMCR- 1997/02/02
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
PHST- 1997/02/02 00:00 [pmc-release]
AID - 96354912 [pii]
AID - 10.1083/jcb.134.4.949 [doi]
PST - ppublish
SO  - J Cell Biol. 1996 Aug;134(4):949-61. doi: 10.1083/jcb.134.4.949.