PMID- 8770508
OWN - NLM
STAT- MEDLINE
DCOM- 19961024
LR  - 20210526
IS  - 1071-412X (Print)
IS  - 1098-6588 (Electronic)
IS  - 1071-412X (Linking)
VI  - 3
IP  - 1
DP  - 1996 Jan
TI  - Evaluation of immunoassays for detection of antibodies to human herpesvirus 7.
PG  - 79-83
AB  - An enzyme immunoassay (EIA), an immunoblot assay (IB), and an indirect 
      immunofluorescence assay were developed for detection of human herpesvirus 7 
      (HHV-7) antibodies in human serum. Cross-absorption studies with EIA or IFA using 
      HHV-7 and human herpesvirus 6 (HHV-6) antigens indicated that most human sera 
      contain cross-reactive HHV-6 and HHV-7 antibodies and that the degree of 
      cross-reactivity varies between individual serum specimens. Inhibition of 
      homologous antibody activity by absorption with heterologous virus ranged from 0 
      to 57% by EIA. However, for every sample tested, absorption with homologous virus 
      removed more activity than did heterologous virus. An 89-kDa protein was 
      identified as an HHV-7-specific serologic marker by IB. Activity to this protein 
      was not removed by absorption with HHV-6 antigen. Of the three assays, the EIA 
      was the most sensitive (94%), while the IB was the most specific (94%). 
      Approximately 80% of specimens collected from German adults and children older 
      than 2 years were positive for HHV-7 antibodies by these assays.
FAU - Black, J B
AU  - Black JB
AD  - Division of Viral and Rickettsial Diseases, Centers for Disease Control and 
      Prevention, Atlanta, Georgia 30333, USA. JXB1@ciddvd1.em.cclc.gov
FAU - Schwarz, T F
AU  - Schwarz TF
FAU - Patton, J L
AU  - Patton JL
FAU - Kite-Powell, K
AU  - Kite-Powell K
FAU - Pellett, P E
AU  - Pellett PE
FAU - Wiersbitzky, S
AU  - Wiersbitzky S
FAU - Bruns, R
AU  - Bruns R
FAU - Muller, C
AU  - Muller C
FAU - Jager, G
AU  - Jager G
FAU - Stewart, J A
AU  - Stewart JA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Clin Diagn Lab Immunol
JT  - Clinical and diagnostic laboratory immunology
JID - 9421292
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antibodies, Viral/*analysis/blood
MH  - Child
MH  - Child, Preschool
MH  - Cross Reactions
MH  - Evaluation Studies as Topic
MH  - Female
MH  - Fetal Blood/immunology
MH  - Fluorescent Antibody Technique, Indirect/methods/statistics & numerical data
MH  - Herpesvirus 6, Human/immunology
MH  - Herpesvirus 7, Human/*immunology
MH  - Humans
MH  - Immunoassay/*methods/statistics & numerical data
MH  - Immunoblotting/methods/statistics & numerical data
MH  - Immunoenzyme Techniques/statistics & numerical data
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - Middle Aged
MH  - Pregnancy
MH  - Sensitivity and Specificity
MH  - Seroepidemiologic Studies
PMC - PMC170251
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
PMCR- 1996/01/01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
PHST- 1996/01/01 00:00 [pmc-release]
AID - 10.1128/cdli.3.1.79-83.1996 [doi]
PST - ppublish
SO  - Clin Diagn Lab Immunol. 1996 Jan;3(1):79-83. doi: 10.1128/cdli.3.1.79-83.1996.