PMID- 8770980
OWN - NLM
STAT- MEDLINE
DCOM- 19961203
LR  - 20190725
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 49
IP  - 1
DP  - 1996 Jan
TI  - Immunolocalization of NAD-dependent 11 beta-hydroxysteroid dehydrogenase in human 
      kidney and colon.
PG  - 271-81
AB  - The inactivation of physiological glucocorticoids by 11 beta-hydroxysteroid 
      dehydrogenase (11 beta-HSD) confers mineralocorticoid specificity to certain 
      aldosterone target tissues. Both NADP, and NAD-dependent isoforms of 11 beta-HSD 
      have been described. An NAD-dependent isoform of 11 beta-HSD (11 beta-HSD2) was 
      recently cloned from human kidney. The present studies were designed to examine 
      the cellular distribution of 11 beta-HSD2 in human kidney and colon, and to 
      determine if the cellular distribution of 11 beta-HSD2 within the human kidney 
      and colon is consistent with a role in conferring mineralocorticoid specificity. 
      Using antibodies against a fusion protein containing a portion of the human 11 
      beta-HSD2, immunohistochemical staining of human kidney showed intense, specific 
      staining of connecting tubules and cortical and medullary collecting tubules and 
      less intense staining in the cortical thick ascending limb. No immunoreactivity 
      was found in proximal tubules, glomeruli, or blood vessels. Within the collecting 
      tubules staining was heterogeneous. The majority of cells showed intense 
      cytoplasmic staining while alpha-intercalated cells displayed much less 
      immunoreactivity. Within the colon, 11 beta-HSD2 immunoreactivity was found 
      predominantly in surface epithelial cells but not in submucosal tissues. Thus, 
      the distribution of the cloned NAD-dependent 11 beta-HSD2 parallels the 
      distribution of mineralocorticoid receptors within the kidney and colon. These 
      results support the view that the NAD-dependent isoform of 11 beta-HSD (11 
      beta-HSD2) provides mineralocorticoid specificity by inactivating glucocorticoids 
      in an autocrine fashion.
FAU - Kyossev, Z
AU  - Kyossev Z
AD  - Division of Nephrology, University of Arkansas for Medical Sciences, Little Rock, 
      USA.
FAU - Walker, P D
AU  - Walker PD
FAU - Reeves, W B
AU  - Reeves WB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0U46U6E8UK (NAD)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Animals
MH  - Base Sequence
MH  - Colonic Neoplasms/*enzymology/pathology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Hydroxysteroid Dehydrogenases/*metabolism
MH  - Immunohistochemistry
MH  - Kidney Neoplasms/*enzymology/pathology
MH  - Molecular Sequence Data
MH  - NAD/*metabolism
MH  - Rabbits
MH  - Tumor Cells, Cultured
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - S0085-2538(15)59326-5 [pii]
AID - 10.1038/ki.1996.39 [doi]
PST - ppublish
SO  - Kidney Int. 1996 Jan;49(1):271-81. doi: 10.1038/ki.1996.39.