PMID- 8773591
OWN - NLM
STAT- MEDLINE
DCOM- 19961017
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 60
IP  - 2
DP  - 1996 Aug
TI  - Cloning of the murine eosinophil peroxidase gene (mEPO): characterization of a 
      conserved subgroup of mammalian hematopoietic peroxidases.
PG  - 285-94
AB  - The mouse eosinophil peroxidase (mEPO) gene was cloned by screening a 
      random-primed bone marrow cDNA library at reduced criteria using a hEPO cDNA. An 
      mEPO cDNA was subsequently used to isolate the mEPO gene from a lambda-genomic 
      library. The mEPO gene displays a high degree of conservation with its human 
      homologue: the transcription units are approximately the same size, conserve the 
      relative size and position of the 12 exons associated with each gene, and at a 
      nucleotide level the mouse and human EPO genes are 86% identical in the protein 
      coding regions and 66% identical in the 3'-untranslated trailer regions. This 
      strong conservation extends to the encoded proteins which show approximately 90% 
      amino acid identity. Expression of the mEPO gene is restricted to tissues 
      containing eosinophil progenitor cells (e.g., bone marrow and spleen), a pattern 
      similar to the expression of another murine eosinophil granule protein, major 
      basic protein.
FAU - Horton, M A
AU  - Horton MA
AD  - Department of Biochemistry and Molecular Biology, Mayo Clinic Scottsdale, 
      Arizona, USA.
FAU - Larson, K A
AU  - Larson KA
FAU - Lee, J J
AU  - Lee JJ
FAU - Lee, N A
AU  - Lee NA
LA  - eng
SI  - GENBANK/L77979
PT  - Journal Article
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Cyanides)
RN  - 0 (DNA, Complementary)
RN  - EC 1.11.1.- (Eosinophil Peroxidase)
RN  - EC 1.11.1.- (Peroxidases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Bone Marrow/enzymology
MH  - Cloning, Molecular
MH  - *Conserved Sequence
MH  - Cyanides/pharmacology
MH  - DNA, Complementary/genetics
MH  - Eosinophil Peroxidase
MH  - Eosinophils/enzymology
MH  - Evolution, Molecular
MH  - Female
MH  - Gene Expression
MH  - Hematopoietic Stem Cells/*enzymology
MH  - Humans
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Peroxidases/biosynthesis/*genetics
MH  - Regulatory Sequences, Nucleic Acid
MH  - Sequence Homology, Amino Acid
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
AID - 10.1002/jlb.60.2.285 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1996 Aug;60(2):285-94. doi: 10.1002/jlb.60.2.285.