PMID- 8774571
OWN - NLM
STAT- MEDLINE
DCOM- 19961010
LR  - 20190611
IS  - 0140-6736 (Print)
IS  - 0140-6736 (Linking)
VI  - 348
IP  - 9027
DP  - 1996 Aug 31
TI  - Association between genetic variants of mast-cell chymase and eczema.
PG  - 581-3
AB  - BACKGROUND: Atopy is a common syndrome underlying asthma, rhinitis, and eczema, 
      and is characterised by high immunoglobulin E (IgE) responses to common antigens. 
      IgE and mast-cell chymase (MCC-a serine protease secreted by skin mast cells) 
      have a key role in atopic or allergic inflammation of the skin. The gene for MCC 
      is located within a cluster of genes for cellular proteases on chromosome 
      14q11.2. We aimed to identify variants of MCC and another gene within this 
      complex, and assess whether there is a genetic association between variants of 
      MCC and atopic disorders-particularly eczema. METHODS: We randomly selected 100 
      controls and recruited patients-100 in each group-with atopic asthma, non-atopic 
      asthma, atopic rhinitis, and atopic eczema. PCR amplification was used to test 
      genomic DNA for an association between allelic polymorphisms in MCC and a 
      flanking gene (CGL1, for the cathepsin-G-like protein) on chromosome 14q11 and 
      asthma, rhinitis, and eczema. FINDINGS: We found a significant association 
      between a BstXI polymorphism in MCC and eczema (odds ratio 2.17 [95% CI 
      1.21-3.88], p = 0.009), but no association with atopic asthma, rhinitis, or 
      non-atopic asthma. There was no association between an Mboll polymorphism in CGL1 
      and any of the atopic disorders. INTERPRETATION: These findings suggest that 
      variants of MCC may be one source of genetic risk for eczema.
FAU - Mao, X Q
AU  - Mao XQ
AD  - Department of Hygeine and Preventive Medicine, Osaka University School of 
      Medicine, Suita, Japan.
FAU - Shirakawa, T
AU  - Shirakawa T
FAU - Yoshikawa, T
AU  - Yoshikawa T
FAU - Yoshikawa, K
AU  - Yoshikawa K
FAU - Kawai, M
AU  - Kawai M
FAU - Sasaki, S
AU  - Sasaki S
FAU - Enomoto, T
AU  - Enomoto T
FAU - Hashimoto, T
AU  - Hashimoto T
FAU - Furuyama, J
AU  - Furuyama J
FAU - Hopkin, J M
AU  - Hopkin JM
FAU - Morimoto, K
AU  - Morimoto K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Lancet
JT  - Lancet (London, England)
JID - 2985213R
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.39 (Chymases)
SB  - IM
EIN - Lancet 1997 Jan 4;349(9044):64
CIN - Lancet. 1996 Aug 31;348(9027):560-1. PMID: 8774564
MH  - Adolescent
MH  - Adult
MH  - Asthma/genetics
MH  - Autoradiography
MH  - Base Sequence
MH  - Case-Control Studies
MH  - Chymases
MH  - Eczema/*genetics
MH  - Female
MH  - Genetic Linkage
MH  - Genetic Variation
MH  - Genotype
MH  - Humans
MH  - Hypersensitivity, Immediate/*genetics
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Random Allocation
MH  - Rhinitis/genetics
MH  - Serine Endopeptidases/*genetics
EDAT- 1996/08/31 00:00
MHDA- 1996/08/31 00:01
CRDT- 1996/08/31 00:00
PHST- 1996/08/31 00:00 [pubmed]
PHST- 1996/08/31 00:01 [medline]
PHST- 1996/08/31 00:00 [entrez]
AID - S0140673695102442 [pii]
AID - 10.1016/s0140-6736(95)10244-2 [doi]
PST - ppublish
SO  - Lancet. 1996 Aug 31;348(9027):581-3. doi: 10.1016/s0140-6736(95)10244-2.