PMID- 8776820
OWN - NLM
STAT- MEDLINE
DCOM- 19961106
LR  - 20180217
IS  - 0042-1138 (Print)
IS  - 0042-1138 (Linking)
VI  - 56
IP  - 4
DP  - 1996
TI  - Efficacy of low-molecular-weight heparin and unfractionated heparin to prevent 
      adhesion of human prostate and bladder carcinoma and melanoma cells to bovine 
      endothelial monolayers. An in vitro study and review of the literature.
PG  - 230-3
AB  - Adherence of human prostate carcinoma (PC 3, DU 145), bladder (647 V, J 82) 
      carcinoma and melanoma (RPMI-8252) cell lines to plastic and bovine endothelial 
      monolayers (BEM) was tested in the presence of 0.5, 1, 5, 10, 20 IU of 
      low-molecular-weight heparin (LMWH), unfractionated heparin (UFH) or saline as a 
      control. Culture medium was supplemented either with 5% fetal calf serum or with 
      human plasma. Floating tumor cells were counted after 2, 4, 24 h of culture in 
      microtiter plates without BEM and after 1.5, 3 and 24 h of culture on BEM. Twenty 
      IU of LMWH increased the numbers of bladder carcinoma cells adhering to BEM as 
      did 20 IU of UFH in cultures of DU 145 prostate carcinoma and RPMI-8252 melanoma 
      cells. LMWH obviously does not prevent human prostate and bladder carcinoma and 
      melanoma cells from adhering to BEM more effectively than UFH. The in vivo effect 
      of UFH and perhaps also LMWH that hinders intravenous tumor cell colonization in 
      blood vessels obviously does not depend on the interaction with endothelial cells 
      alone.
FAU - Lersch, C
AU  - Lersch C
AD  - Second Department of Internal Medicine, Technical University of Munich, Germany.
FAU - Gericke, D
AU  - Gericke D
FAU - Classen, M
AU  - Classen M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Urol Int
JT  - Urologia internationalis
JID - 0417373
RN  - 0 (Anticoagulants)
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Anticoagulants/*pharmacology
MH  - Carcinoma/*metabolism/pathology
MH  - Cattle
MH  - Cell Adhesion/drug effects
MH  - Endothelium, Vascular/*physiology
MH  - Heparin/*pharmacology
MH  - Heparin, Low-Molecular-Weight/*pharmacology
MH  - Humans
MH  - Male
MH  - Melanoma/*metabolism/pathology
MH  - Prostatic Neoplasms/*metabolism/pathology
MH  - Tumor Cells, Cultured
MH  - Urinary Bladder Neoplasms/*metabolism/pathology
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1159/000282848 [doi]
PST - ppublish
SO  - Urol Int. 1996;56(4):230-3. doi: 10.1159/000282848.