PMID- 8776977
OWN - NLM
STAT- MEDLINE
DCOM- 19961204
LR  - 20190826
IS  - 0030-6622 (Print)
IS  - 0030-6622 (Linking)
VI  - 99
IP  - 7
DP  - 1996 Jul
TI  - [Combined fluorescence in situ hybridization and cytofluorometric analysis of 
      numerical chromosomal aberrations in squamous cell carcinomas of the tongue].
PG  - 1026-35
AB  - DNA cytofluorometry and fluorescence in situ hybridization (FISH) with 
      centromeric repetitive probes of chromosomes 1, 7, 11, 17, X and Y were used to 
      detect numerical chromosomal aberrations in 13 squamous cell carcinomas of the 
      tongue. In 4 of the 6 DNA-diploid tumors examined, significant numerical 
      aberrations in at least 1 of the chromosomes were detected. The main line of 1 
      tumor showed trisomy 1 and the other 3 tumors had significant sublines that 
      showed trisomy or monosomy of the 1 or 2 chromosomes examined. No common specific 
      aberrations were detected in these tumors. Cytofluorometrically we found 
      polyploidy in all 4 of the tumors with numerical chromosomal aberrations. These 
      results suggest that subpopulations which are aneuploid at the chromosome level 
      arise preferentially from DNA-diploid tumors with polyploidy before the ploidy of 
      the main line shifts to overt DNA-aneuploidy. In all of the 7 DNA-aneuploid 
      tumors examined, we detected numerical chromosomal aberrations. Most of the 
      aberrations were thought to occur after tetraploidization because a gain in 
      chromosomal copy number including tetrasomy was common. In 3 DNA-aneuploid 
      tumors, however, the main line showed disomy 11, whereas the other chromosomes 
      examined had 3 or more copies. In 1 of the 3 tumors there was a significant 
      subline of monosomy 11. In these 3 tumors disomy 11 may have been preceded by a 
      DNA-diploid stage with monosomy 11 before tetraploidization.
FAU - Nakai, S
AU  - Nakai S
AD  - Department of Otolaryngology, Kyoto Prefectural University of Medicine.
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Nihon Jibiinkoka Gakkai Kaiho
JT  - Nihon Jibiinkoka Gakkai kaiho
JID - 7505728
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics
MH  - *Chromosome Aberrations
MH  - DNA, Neoplasm/analysis
MH  - *Flow Cytometry
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Ploidies
MH  - Tongue Neoplasms/*genetics
EDAT- 1996/07/01 00:00
MHDA- 1996/07/01 00:01
CRDT- 1996/07/01 00:00
PHST- 1996/07/01 00:00 [pubmed]
PHST- 1996/07/01 00:01 [medline]
PHST- 1996/07/01 00:00 [entrez]
AID - 10.3950/jibiinkoka.99.1026 [doi]
PST - ppublish
SO  - Nihon Jibiinkoka Gakkai Kaiho. 1996 Jul;99(7):1026-35. doi: 
      10.3950/jibiinkoka.99.1026.