PMID- 8778029
OWN - NLM
STAT- MEDLINE
DCOM- 19960916
LR  - 20190512
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 87
IP  - 3
DP  - 1996 Mar
TI  - The bacterial superantigen Staphylococcal enterotoxin B stimulates lymphocyte 
      locomotor capacity during culture in vitro.
PG  - 428-33
AB  - The bacterial superantigen Staphylococcal enterotoxin B (SEB) was investigated 
      for its effects on lymphocyte locomotion in vitro. Culture of peripheral blood 
      mononuclear cells (PBMC) for 24-72 hr in SEB (1-100 micrograms/ml) increased the 
      proportion of lymphocytes in locomotor (polarized) morphology and capable of 
      invading collagen gels, to the same extent as the established locomotor 
      activator, anti-CD3 (alpha-CD3), though the conventional antigen, tetanus toxoid 
      was ineffective. The cells responding to SEB were predominantly T cells. SEB had 
      no effect on lymphocyte locomotion in short-term (45 min) assays, thus its effect 
      is to stimulate growth-related locomotor capacity and it does not act as a 
      chemoattractant. During culture of PBMC in SEB, the chemokines interleukin-8 
      (IL-8) and macrophage chemotactic protein-1 (MCP-1) were released into the 
      culture medium. The presence of anti-IL-8, but not of anti-MCP-1, either during 
      culture or added to SEB culture supernatants and tested in short-term assays, 
      inhibited the development of polarization suggesting that IL-8, which is a 
      lymphocyte chemoattractant, also plays a key role in SEB-induced locomotor 
      activation. Among SEB-activated lymphocytes, CD45RO+CD45RA- lymphocytes showed 
      enhanced locomotor responses, but a relation was not found between locomotor 
      activity and the presence of cell surface CD69.
FAU - Newman, I
AU  - Newman I
AD  - Department of Immunology, University of Glasgow, UK.
FAU - Wilkinson, P C
AU  - Wilkinson PC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Chemokine CCL2)
RN  - 0 (Enterotoxins)
RN  - 0 (Interleukin-8)
RN  - 0 (Superantigens)
RN  - 39424-53-8 (enterotoxin B, staphylococcal)
SB  - IM
MH  - Cell Movement/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Electrophoresis
MH  - Enterotoxins/*pharmacology
MH  - Humans
MH  - Immunophenotyping
MH  - Interleukin-8/metabolism
MH  - Leukocytes, Mononuclear/*drug effects/physiology
MH  - Lymphocyte Activation
MH  - *Staphylococcus aureus
MH  - Superantigens/*pharmacology
MH  - T-Lymphocytes/drug effects/physiology
PMC - PMC1384112
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
PMCR- 1997/03/01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
PHST- 1997/03/01 00:00 [pmc-release]
AID - 10.1046/j.1365-2567.1996.491565.x [doi]
PST - ppublish
SO  - Immunology. 1996 Mar;87(3):428-33. doi: 10.1046/j.1365-2567.1996.491565.x.