PMID- 8779919
OWN - NLM
STAT- MEDLINE
DCOM- 19961220
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 270
IP  - 2 Pt 1
DP  - 1996 Feb
TI  - Regulation of ICAM-1 by dexamethasone in a human vascular endothelial cell line 
      EAhy926.
PG  - C552-61
AB  - Regulation by dexamethasone of intercellular adhesion molecule-1 (ICAM-1) in 
      cultured monolayers of the human umbilical vein endothelial cell line EAhy926 was 
      investigated. Tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma 
      (IFN-gamma) in combination or lipopolysaccharide (LPS) alone gave time- and 
      dose-dependent increases in ICAM-1. Sustained expression of ICAM-1 was observed 
      after short exposure (30 min) to TNF-alpha + IFN-gamma, but not to LPS. 
      LPS-induced ICAM-1 expression was not inhibited by interleukin-1 (IL-1) receptor 
      antagonist (0.01-100 micrograms/ml). Dexamethasone (1,000 nM) did not inhibit 
      TNF-alpha + IFN-gamma-induced ICAM-1 expression or mRNA induction. In contrast, 
      dexamethasone dose dependently (0.1-1,000 nM) inhibited LPS-induced ICAM-1 
      expression; however, its effect on mRNA was not established, because ICAM-1 mRNA 
      induced by LPS was not detected at the time points investigated in this study (3 
      and 20 h). Adhesion of unstimulated human neutrophils to EAhy926 monolayers 
      activated with TNF-alpha + IFN-gamma or LPS was increased in the presence of 
      dexamethasone at low doses, whereas neutrophil adhesion to LPS- but not 
      cytokine-stimulated endothelial cells was significantly reduced (P < 0.05) in the 
      presence of a high dose of dexamethasone (1,000 nM). In conclusion, dexamethasone 
      was demonstrated to regulate the expression and function of ICAM-1 in a 
      stimulus-dependent manner.
FAU - Burke-Gaffney, A
AU  - Burke-Gaffney A
AD  - Department of Applied Pharmacology, National Heart and Lung Institute, London, 
      United Kingdom.
FAU - Hellewell, P G
AU  - Hellewell PG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Cell Line
MH  - Cytokines/pharmacology
MH  - Dexamethasone/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Endothelium, Vascular/cytology/*metabolism
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/genetics/*metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - RNA, Messenger/metabolism
MH  - Time Factors
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
AID - 10.1152/ajpcell.1996.270.2.C552 [doi]
PST - ppublish
SO  - Am J Physiol. 1996 Feb;270(2 Pt 1):C552-61. doi: 10.1152/ajpcell.1996.270.2.C552.