PMID- 8780379
OWN - NLM
STAT- MEDLINE
DCOM- 19961017
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 149
IP  - 3
DP  - 1996 Sep
TI  - Genetic heterogeneity of primary and metastatic breast carcinoma defined by 
      fluorescence in situ hybridization.
PG  - 751-8
AB  - Breast carcinoma is frequently associated with nonrandom chromosomal aberrations, 
      but their identification by standard cytogenetics (SC) is often limited by 
      technical difficulties. Fluorescence in situ hybridization (FISH) studies of 
      interphase nuclei can circumvent some of these difficulties and has the potential 
      to identify nonrandom molecular cytogenetic events occurring in breast cancer. 
      FISH was performed on tumor nuclei isolated from 15 formalin-fixed, 
      paraffin-embedded archival breast carcinomas using a panel of chromosome-specific 
      alpha-satellite probes for enumerating chromosomes in interphase nuclei. Freshly 
      isolated cells from these same cases had previously been studied by standard 
      cytogenetics and FISH. In addition to archival primary carcinoma, archival 
      metastases and normal tissue were also studied by FISH. Genetic numerical 
      alterations were identified by standard cytogenetics or FISH in 14 of 15 
      carcinomas. Numeric alterations initially identified by standard cytogenetics 
      were confirmed by FISH in 9 of 10 cases. Results of FISH performed on nuclei 
      isolated from paraffin-embedded material were in agreement with FISH performed on 
      freshly isolated cells. Clonal numeric alterations were observed in the archival 
      primary tumor as well as in metastases. Archival normal tissue was consistently 
      disomic.
FAU - Simpson, J F
AU  - Simpson JF
AD  - Division of Pathology, City of Hope National Medical Center, Duarte, California 
      91010, USA.
FAU - Quan, D E
AU  - Quan DE
FAU - Ho, J P
AU  - Ho JP
FAU - Slovak, M L
AU  - Slovak ML
LA  - eng
GR  - CA33572/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Breast Neoplasms/chemistry/*genetics/pathology
MH  - Carcinoma/chemistry/*genetics/pathology/secondary
MH  - Cell Nucleus/chemistry
MH  - Chromosome Aberrations
MH  - Female
MH  - Formaldehyde
MH  - *Genetic Heterogeneity
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Lymph Nodes/chemistry/cytology
MH  - Middle Aged
MH  - Paraffin Embedding
MH  - Tumor Cells, Cultured
PMC - PMC1865143
EDAT- 1996/09/01 00:00
MHDA- 1996/09/01 00:01
PMCR- 1997/03/01
CRDT- 1996/09/01 00:00
PHST- 1996/09/01 00:00 [pubmed]
PHST- 1996/09/01 00:01 [medline]
PHST- 1996/09/01 00:00 [entrez]
PHST- 1997/03/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1996 Sep;149(3):751-8.