PMID- 8780745
OWN - NLM
STAT- MEDLINE
DCOM- 19961016
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 90
IP  - 1
DP  - 1996 Aug
TI  - Combined cytogenetic and molecular genetic analyses of fifty-nine untreated human 
      prostate carcinomas.
PG  - 37-44
AB  - G-banding analyses and molecular genetic investigations (fluorescence in situ 
      hybridization (FISH) and loss of heterozygosity (LOH) studies) were performed in 
      59 tumor and nontumorous samples of human prostate carcinoma. Clonal chromosome 
      aberrations were detected in 16 tumors of which nine were poorly differentiated 
      (G3) and 11 in an advanced stage (pT3). Six cases showed numerical chromosome 
      aberrations. The most common numerical aberrations were trisomy 7 and loss of the 
      Y chromosome each present in three tumors. Clonal structural aberrations were 
      detected in 12 tumors. Deletions could be observed in two cases affecting 
      chromosome 6q23 and in two cases affecting chromosomal region 16q. A structural 
      variant of the pericentromeric heterochromatin of chromosome 9 became apparent in 
      six cases. The Y chromosome was involved in clonal translocations in two cases, 
      additionally an inversion occurred on chromosome 19 in one case. All clonal 
      chromosomal changes were found exclusively in the tumor sample. For an analysis 
      of the pericentromeric heterochromatin of chromosome 9, FISH using a chromosome 
      9-specific sat III DNA probe was carried out on metaphase preparations of tumor 
      and nontumorous tissues of two cases showing var(9)(qh). The FISH data suggest a 
      deletion in the pericentromeric heterochromatin. Loss of heterozygosity studies 
      on chromosomal regions 10q and 16q were carried out because both chromosomes were 
      frequently affected by nonclonal structural aberrations. Loss of heterozygosity 
      could be verified in 11 cases.
FAU - Zitzelsberger, H
AU  - Zitzelsberger H
AD  - Institut fur Strahlenbiologie, GSF-Forschungszentrum fur Umwelt und Gesundheit 
      GmbH, Munchen, Germany.
FAU - Szucs, S
AU  - Szucs S
FAU - Robens, E
AU  - Robens E
FAU - Weier, H U
AU  - Weier HU
FAU - Hofler, H
AU  - Hofler H
FAU - Bauchinger, M
AU  - Bauchinger M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Heterochromatin)
SB  - IM
MH  - Aged
MH  - Aneuploidy
MH  - Base Sequence
MH  - Carcinoma/*genetics/pathology
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - Chromosomes, Human/ultrastructure
MH  - Heterochromatin/ultrastructure
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Prostatic Neoplasms/*genetics/pathology
MH  - Retrospective Studies
MH  - Sequence Deletion
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
AID - 0165460896000659 [pii]
AID - 10.1016/0165-4608(96)00065-9 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1996 Aug;90(1):37-44. doi: 10.1016/0165-4608(96)00065-9.