PMID- 8781994
OWN - NLM
STAT- MEDLINE
DCOM- 19961120
LR  - 20191101
IS  - 0948-6143 (Print)
IS  - 0948-6143 (Linking)
VI  - 105
IP  - 5
DP  - 1996 May
TI  - Detection of numerical karyotype changes in the giant cells of Hodgkin's 
      lymphomas by a combination of FISH and immunohistochemistry applied to paraffin 
      sections.
PG  - 401-4
AB  - Conventional karyotyping of Hodgkin's disease (HD) has until now yielded only 
      limited insight into karyotypic characteristics of this disease. For this reason, 
      fluorescence in situ hybridization (FISH) using alpha-satellite 
      chromosome-specific probes was applied to paraffin sections of HD tumors in order 
      to verify numerical aberrations suggested to be specific for HD in the 
      literature. The FISH technique was combined with immunohistochemical detection of 
      the CD30 antigen to allow easier identification of the Reed-Sternberg and Hodgkin 
      (RS&H) cells. The number of specific FISH signals per nucleus was determined both 
      in CD30-positive RS&H cells as well as in non-malignant "bystander" cells in 
      order to assess differences in the signal distribution. Contrasted with normal 
      lymphoid cells, the tumor cells in HD were found to be polysomic for at least one 
      of the chromosomes analyzed (1,2,4, and 8). The technique described is a reliable 
      method for confirmation of results obtained from conventional cytogenetics, which 
      is especially suited for archival material or samples not containing dividing 
      cells.
FAU - Nolte, M
AU  - Nolte M
AD  - Pathologisches Institut der Medizinischen Hochschule Hannover, Germany.
FAU - Werner, M
AU  - Werner M
FAU - von Wasielewski, R
AU  - von Wasielewski R
FAU - Nietgen, G
AU  - Nietgen G
FAU - Wilkens, L
AU  - Wilkens L
FAU - Georgii, A
AU  - Georgii A
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Histochem Cell Biol
JT  - Histochemistry and cell biology
JID - 9506663
RN  - 0 (Antigens)
SB  - IM
MH  - Antigens/analysis
MH  - *Chromosome Aberrations
MH  - Hodgkin Disease/*genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping/*methods
MH  - Palatine Tonsil/ultrastructure
MH  - Paraffin Embedding
MH  - Reed-Sternberg Cells/ultrastructure
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - 10.1007/BF01463661 [doi]
PST - ppublish
SO  - Histochem Cell Biol. 1996 May;105(5):401-4. doi: 10.1007/BF01463661.