PMID- 8782359
OWN - NLM
STAT- MEDLINE
DCOM- 19961107
LR  - 20190702
IS  - 0042-4900 (Print)
IS  - 0042-4900 (Linking)
VI  - 138
IP  - 22
DP  - 1996 Jun 1
TI  - Exposure of gilts in early gestation to porcine reproductive and respiratory 
      syndrome virus.
PG  - 536-9
AB  - Twenty-five gilts without measurable serum antibody titres to porcine 
      reproductive and respiratory syndrome virus (PRRSV) were identified and 16 were 
      inoculated with PRRSV at seven, 14 or 21 days of gestation and killed 20 to 22 
      days later to determine the effect of the virus on their embryos. The remaining 
      nine gilts were not exposed to PRRSV, but were killed at the same stages of 
      gestation. The gilts were observed for clinical signs of infection and the gilts 
      and their embryos were tested for PRRSV and homologous antibodies. The infection 
      was demonstrated by the re-isolation of the virus and its detection by the 
      reverse transcriptase polymerase chain reaction in serum and other tissue samples 
      from the inoculated gilts, and also by seroconversion. However, the gilts 
      remained healthy throughout the study, except for one which was depressed and 
      anorexic for two days. Two of the litters from the gilts challenged with PRRSV on 
      day 14 of gestation contained one and three infected live embryos; the other 
      embryos from these two litters did not contain detectable virus, although most of 
      the embryos in one of the litters were dead. The other nine litters from the 
      gilts challenged with PRRSV and the control litters, showed no evidence of 
      infection.
FAU - Prieto, C
AU  - Prieto C
AD  - Departamento de Patologia Animal 1, Facultad de Veterinaria, Universidad 
      Complutense de Madrid, Spain.
FAU - Sanchez, R
AU  - Sanchez R
FAU - Martin-Rillo, S
AU  - Martin-Rillo S
FAU - Suarez, P
AU  - Suarez P
FAU - Simarro, I
AU  - Simarro I
FAU - Solana, A
AU  - Solana A
FAU - Castro, J M
AU  - Castro JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Vet Rec
JT  - The Veterinary record
JID - 0031164
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Embryo, Mammalian/*virology
MH  - Estrus/physiology
MH  - Female
MH  - Infectious Disease Transmission, Vertical/*veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/transmission/*virology
MH  - Porcine respiratory and reproductive syndrome virus/immunology
MH  - Pregnancy
MH  - Pregnancy Complications, Infectious/immunology/*veterinary/virology
MH  - Swine/immunology/*virology
MH  - Time Factors
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
AID - 10.1136/vr.138.22.536 [doi]
PST - ppublish
SO  - Vet Rec. 1996 Jun 1;138(22):536-9. doi: 10.1136/vr.138.22.536.