PMID- 8782433
OWN - NLM
STAT- MEDLINE
DCOM- 19961114
LR  - 20190606
IS  - 0018-0661 (Print)
IS  - 0018-0661 (Linking)
VI  - 124
IP  - 2
DP  - 1996
TI  - Mouse euchromatin specific "genome-painting" with a LINE probe: a rapid method 
      for identification and mapping of human chromosomes in mouse-human microcell 
      hybrids by two-color FISH.
PG  - 131-5
AB  - We describe the use of a long interspersed repetitive sequence (mCPE1.51) for 
      mouse euchromatin specific "genome-painting". In fluorescence in situ 
      hybridization (FISH) experiments, this probe was suitable for identification of 
      the mouse genome and disclosure of translocations of mouse chromosome segments to 
      chromosomes of different species without suppression hybridization. The 
      euchromatin specificity of the probe allowed the discrimination between 
      euchromatin and heterochromatin of mouse chromosomes. Simultaneous hybridization 
      of the biotinylated mouse specific genome-painting probe and a 
      digoxigenin-labeled human chromosome 3-specific cosmid probe to metaphase spreads 
      of mouse-human microcell hybrid carrying a single deleted human chromosome 3 on a 
      mouse fibrosarcoma background, allowed rapid identification and mapping of human 
      chromosome 3.
FAU - Katona, R
AU  - Katona R
AD  - Institute of Genetics, Hungarian Academy of Sciences, Szeged, Hungary.
FAU - Szeles, A
AU  - Szeles A
FAU - Hadlaczky, G
AU  - Hadlaczky G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hereditas
JT  - Hereditas
JID - 0374654
RN  - 0 (Chromatin)
RN  - 0 (DNA Probes)
SB  - IM
MH  - Animals
MH  - Chromatin/*physiology
MH  - Chromosome Mapping
MH  - DNA Probes
MH  - *Genome, Human
MH  - Humans
MH  - Hybrid Cells/*physiology
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Time Factors
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1111/j.1601-5223.1996.00131.x [doi]
PST - ppublish
SO  - Hereditas. 1996;124(2):131-5. doi: 10.1111/j.1601-5223.1996.00131.x.