PMID- 8783159
OWN - NLM
STAT- MEDLINE
DCOM- 19961205
LR  - 20190830
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 58
IP  - 1-2
DP  - 1996 Apr 26
TI  - A method for detection of HHV-6 antigens and its use for evaluating antiviral 
      drugs.
PG  - 137-43
AB  - A simple and reproducible method for detection of human herpesvirus 6 (HHV-6) 
      antigens was developed using a dot blot assay in order to assess virus titer and 
      to evaluate the effect of antiviral drugs against HHV-6. The titer of virus 
      stocks obtained by the dot blot assay was the same as that determined by an 
      immunofluorescence assay (IFA). This method was then applied to evaluate the 
      effect of several antiviral drugs against HHV-6, including phosphonoformic acid 
      (PFA), 9-(2-hydroxyethoxymethyl)guanine (ACV), 
      9-[(1,3-dihydroxy-2-propoxy)methyl]guanine (DHPG) and 
      (S)-1-[(3-hydroxy-2-phosphonylmethoxy)propyl]cytosine [(S)-HPMPC]. The end-point 
      concentrations (EPC, which was determined visually) of DHPG and (S)-HPMPC were 
      approximately 1 microgram/ml. These drugs were more effective than the others 
      which had EPCs of approximately 16 micrograms/ml each. The EPC values of four 
      drugs were almost similar to EC90 values determined by measuring density of each 
      dot blot. Thus, the EPC values can be utilized to determine the efficacy of these 
      drugs in the inhibition of HHV-6 replication. The block in virus replication was 
      not due to toxic effect of these drugs on cord blood mononuclear cells (CBMCs). 
      These results suggest that a dot blot method which detects HHV-6 antigens can be 
      useful for titrating virus yield and evaluating antiviral drugs against HHV-6 
      replication.
FAU - Yoshida, M
AU  - Yoshida M
AD  - Department of Pediatrics, University of Alabama at Birmingham 35294, USA.
FAU - Yamada, M
AU  - Yamada M
FAU - Chatterjee, S
AU  - Chatterjee S
FAU - Lakeman, F
AU  - Lakeman F
FAU - Nii, S
AU  - Nii S
FAU - Whitley, R J
AU  - Whitley RJ
LA  - eng
GR  - N0I-AI-12667/AI/NIAID NIH HHS/United States
GR  - N0I-AI-15113/AI/NIAID NIH HHS/United States
GR  - RR-032/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Antigens, Viral)
RN  - 0 (Antiviral Agents)
RN  - 0 (Organophosphonates)
RN  - 0 (Organophosphorus Compounds)
RN  - 364P9RVW4X (Foscarnet)
RN  - 8J337D1HZY (Cytosine)
RN  - 96429-66-2 (9-((2,3-dihydroxy-1-propoxy)methyl)guanine)
RN  - JIL713Q00N (Cidofovir)
RN  - P9G3CKZ4P5 (Ganciclovir)
SB  - IM
MH  - Antigens, Viral/*analysis
MH  - Antiviral Agents/*pharmacology/toxicity
MH  - Cidofovir
MH  - Cytosine/*analogs & derivatives/pharmacology/toxicity
MH  - Drug Evaluation
MH  - Foscarnet/*pharmacology/toxicity
MH  - Ganciclovir/*analogs & derivatives/pharmacology/toxicity
MH  - Herpesvirus 6, Human/*drug effects/immunology
MH  - Humans
MH  - *Organophosphonates
MH  - Organophosphorus Compounds/*pharmacology/toxicity
MH  - Reproducibility of Results
EDAT- 1996/04/26 00:00
MHDA- 1996/04/26 00:01
CRDT- 1996/04/26 00:00
PHST- 1996/04/26 00:00 [pubmed]
PHST- 1996/04/26 00:01 [medline]
PHST- 1996/04/26 00:00 [entrez]
AID - 0166-0934(95)02002-0 [pii]
AID - 10.1016/0166-0934(95)02002-0 [doi]
PST - ppublish
SO  - J Virol Methods. 1996 Apr 26;58(1-2):137-43. doi: 10.1016/0166-0934(95)02002-0.