PMID- 8783248
OWN - NLM
STAT- MEDLINE
DCOM- 19970311
LR  - 20190712
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 73
IP  - 1
DP  - 1996 Jul
TI  - Catechol-O-methyltransferase in rat sensory ganglia and spinal cord.
PG  - 267-76
AB  - The localization of catechol-O-methyltransferase immunoreactivity in rat dorsal 
      root ganglia and in the spinal cord and its co-existence with substance P, 
      calcitonin gene-related peptide and fluoride-resistant acid phosphatase in dorsal 
      root ganglion cells was examined with immunohistochemical and histochemical 
      double-staining methods. Analysis of dorsal of dorsal root ganglia at both 
      cervical and lumbar levels revealed catechol-O-methyltransferase immunoreactivity 
      in numerous dorsal root ganglion cells. Double-staining studies showed that 
      catechol-O-methyltransferase and substance P immunoreactivities were located in 
      different cells with a few exceptions, whereas both catechol-O-methyltransferase 
      and calcitonin gene-related peptide immunoreactivities were detected in about 10% 
      of all labeled cells positive for one of the two markers at both levels studied. 
      The great majority of fluoride-resistant alkaline phosphatase-positive cells were 
      also immunoreactive for catechol-O-methyltransferase. Again, no difference was 
      found between cervical and lumbar levels. Catechol-O-methyltransferase 
      immunoreactivity was also found in the neuropil of the dorsal horn of the spinal 
      cord. The staining was most intense in the superficial laminae (I-III) and 
      overlapped partly with substance P and calcitonin gene-related peptide 
      immunoreactivity. Western blotting analysis revealed that soluble 
      catechol-O-methyltransferase was the clearly dominating form of the enzyme in 
      dorsal root ganglia. The distribution pattern of catechol-O-methyltransferase in 
      dorsal horn and sensory neurons suggests that the enzyme may modulate sensory 
      neurotransmission.
FAU - Karhunen, T
AU  - Karhunen T
AD  - Department of Biology, Abo Akademi University, Turku, Finland.
FAU - Ulmanen, I
AU  - Ulmanen I
FAU - Panula, P
AU  - Panula P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 33507-63-0 (Substance P)
RN  - EC 2.1.1.6 (Catechol O-Methyltransferase)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - EC 3.1.3.2 (Acid Phosphatase)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
SB  - IM
MH  - Acid Phosphatase/metabolism
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Blotting, Western
MH  - Calcitonin Gene-Related Peptide/metabolism
MH  - Catechol O-Methyltransferase/*metabolism
MH  - Ganglia, Sensory/*enzymology/ultrastructure
MH  - Ganglia, Spinal/*enzymology/ultrastructure
MH  - Immunohistochemistry
MH  - Male
MH  - Microscopy, Immunoelectron
MH  - Rats
MH  - Rats, Wistar
MH  - Substance P/metabolism
EDAT- 1996/07/01 00:00
MHDA- 1996/07/01 00:01
CRDT- 1996/07/01 00:00
PHST- 1996/07/01 00:00 [pubmed]
PHST- 1996/07/01 00:01 [medline]
PHST- 1996/07/01 00:00 [entrez]
AID - 0306452296000164 [pii]
AID - 10.1016/0306-4522(96)00016-4 [doi]
PST - ppublish
SO  - Neuroscience. 1996 Jul;73(1):267-76. doi: 10.1016/0306-4522(96)00016-4.