PMID- 8784259
OWN - NLM
STAT- MEDLINE
DCOM- 19961017
LR  - 20220408
IS  - 0165-5728 (Print)
IS  - 0165-5728 (Linking)
VI  - 68
IP  - 1-2
DP  - 1996 Aug
TI  - Induction of immunoreactive interleukin-1 beta and tumor necrosis factor-alpha in 
      the brains of rabies virus infected rats.
PG  - 45-51
AB  - Interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF alpha) are 
      important cytokines in the development of brain inflammation during pathological 
      process. During rabies virus infection, the level of these proinflammatory 
      cytokines are enhanced in the brain. In the present study we determined the 
      cellular localization of these two cytokines by immunocytochemistry in brains of 
      rats infected with rabies virus, at different time-intervals of the disease (day 
      1, 3, 4, 5 and at final stage day 6 post-infection (p.i.)). Cellular 
      identification of IL-1 beta (irIL-1 beta) and TNF alpha (irTNF alpha) 
      immunopositive cells was studied using a polyclonal antibody against these 
      cytokines and against glial fibrillary acidic protein (GFAP) to detect astrocytes 
      and GSA-I-B4 isolectin to detect microglial cells and/or infiltrating 
      macrophages. In brains of control and early infected rats, irIL-1 beta was only 
      detected in fibers located in the hypothalamus, supraoptic and tractus optic 
      nuclei and infundibular nucleus. From day 4 onwards until day 6 p.i., enhanced 
      irIL-1 beta was found and identified either in activated ameboid and/or 
      infiltrated macrophages (amygdala, thalamus, internal capsula, subtantia nigra, 
      septal nuclei and around blood vessels), or in activated ramified cells 
      (hypothalamus and periventricular nucleus, piriformis and cingulate cortex, 
      hippocampus). IrTNF alpha was observed in the brains of rats at a final stage of 
      disease (day 5 and 6 p.i.): in the hypothalamus, the amygdala, the internal 
      capsula, the thalamus, the septal nuclei, the hippocampus, the habenular nuclei 
      and around the blood vessels. Ir-TNF alpha was detected in round cells identified 
      as ameboid microglia and/or infiltrated macrophages. A marked activation of 
      microglial and astroglial cells was observed mainly in the hypothalamus, the 
      thalamus and hippocampus and around the blood vessels, at day 4 p.i. and later, 
      revealing a high central inflammatory reaction in brains of rabies virus infected 
      rats. These results showed that IL-1 beta and TNF alpha are produced in the brain 
      both by local microglial cells and infiltrating macrophages during rabies 
      infection. Thus, these cytokines may play an important role in coordinating the 
      dramatic inflammatory response associated with the rabies-encephalopathy as well 
      as in the neural modification and alteration of brain functions.
FAU - Marquette, C
AU  - Marquette C
AD  - Rabies Unit, Pasteur Institute, Paris, France. chmarq@pasteur.fr
FAU - Van Dam, A M
AU  - Van Dam AM
FAU - Ceccaldi, P E
AU  - Ceccaldi PE
FAU - Weber, P
AU  - Weber P
FAU - Haour, F
AU  - Haour F
FAU - Tsiang, H
AU  - Tsiang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (Interleukin-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Animals, Suckling
MH  - Brain/*immunology/virology
MH  - Brain Chemistry/immunology
MH  - Interleukin-1/*immunology
MH  - Male
MH  - Mice
MH  - Microglia/chemistry/immunology/virology
MH  - Rabbits
MH  - Rabies/*immunology
MH  - Rabies virus/*immunology
MH  - Rats
MH  - Rats, Wistar
MH  - Tumor Necrosis Factor-alpha/*immunology
EDAT- 1996/08/01 00:00
MHDA- 2000/06/01 09:00
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 2000/06/01 09:00 [medline]
PHST- 1996/08/01 00:00 [entrez]
AID - 0165-5728(96)00056-2 [pii]
AID - 10.1016/0165-5728(96)00056-2 [doi]
PST - ppublish
SO  - J Neuroimmunol. 1996 Aug;68(1-2):45-51. doi: 10.1016/0165-5728(96)00056-2.