PMID- 8785614
OWN - NLM
STAT- MEDLINE
DCOM- 19960924
LR  - 20191101
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 4
IP  - 2
DP  - 1996 Feb
TI  - Strukturdifferenzierungen in Y-chromosom von Drosophila hydei: the unique 
      morphology of the Y chromosomal lampbrush loops Threads results from 'coaxial 
      shells' formed by different satellite-specific subregions within megabase-sized 
      transcripts.
PG  - 87-102
AB  - The results of pulsed-field gel electrophoresis (PFGE) analysis and two-colour 
      transcript fluorescence in situ hybridization (FISH) for the three 
      Threads-specific DNA satellites YLII, YLI and rally are in support of long-range 
      clustering of these sequence families within the subterminal region on the long 
      arm of the Y chromosome of Drosophila hydei. On the basis of the linear 
      arrangement of at least four extended clusters of satellite-specific sequences, 
      the loop morphology of wild-type and several mutant Threads can be explained by 
      assumption of a single Threads-specific transcription unit comprising about 5.1 
      Mb of repetitive DNA located between the Pseudonucleolus and the Nucleolus 
      organizer. Transcription is unidirectional from the Pseudonucleolus towards the 
      terminally located Nucleolus organizer. Transcripts most likely start in front of 
      or within the 3.2 Mb region of YLII-related sequences, pass through subsequent 
      blocks of 1.2 and 0.3 Mb of YLI- and rally-related sequences, respectively, and 
      cease within the region of a smaller block of YLI-related repeats. The 
      megabase-sized transcripts remain physically linked to the DNA axis and their 
      extended satellite-specific regions form coaxial clouds or shells around the 
      central DNA axis. In this way each cluster of earlier-transcribed sequences 
      generates a cloud or shell on top of the later-transcribed ones. According to 
      this model of 'satellite-specific coaxial shells' the tube-like morphology and 
      other peculiarities of the Y chromosomal lampbrush loops Threads can be explained 
      as a result of satellite-specific RNA superstructures and/or formation of 
      extended ribonucleoprotein (RNP) complexes between clusters of satellite-specific 
      transcripts and specific proteins. On the basis of this model the specific 
      morphology of several Threads mutants can be interpreted as the result of large 
      interstitial or terminal deletions that alter the total length of the 
      Threads-specific transcription unit without exerting other major effects on 
      principal features of the transcription process along the Threads.
FAU - Kurek, R
AU  - Kurek R
AD  - Institute of Genetics, Heinrich-Heine-Universitat Dusseldorf, Germany.
FAU - Trapitz, P
AU  - Trapitz P
FAU - Bunemann, H
AU  - Bunemann H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
SB  - IM
MH  - Animals
MH  - Blotting, Southern
MH  - Cell Nucleus/genetics
MH  - Drosophila/*genetics/physiology
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Fertility/genetics/physiology
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Microscopy, Phase-Contrast
MH  - Mutation
MH  - Nucleic Acid Hybridization
MH  - Repetitive Sequences, Nucleic Acid
MH  - Sequence Deletion
MH  - Spermatocytes/ultrastructure
MH  - Transcription, Genetic
MH  - Y Chromosome/*genetics/ultrastructure
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
AID - 10.1007/BF02259701 [doi]
PST - ppublish
SO  - Chromosome Res. 1996 Feb;4(2):87-102. doi: 10.1007/BF02259701.