PMID- 8786090
OWN - NLM
STAT- MEDLINE
DCOM- 19960920
LR  - 20220316
IS  - 0340-6717 (Print)
IS  - 0340-6717 (Linking)
VI  - 97
IP  - 3
DP  - 1996 Mar
TI  - High resolution multicolor fluorescence in situ hybridization using cyanine and 
      fluorescein dyes: rapid chromosome identification by directly fluorescently 
      labeled alphoid DNA probes.
PG  - 390-8
AB  - We tested DNA probes directly labeled by fluorescently labeled nucleotides 
      (Cy3-dCTP, Cy5-dCTP, FluorX-dCTP) for high resolution uni- and multicolor 
      detection of human chromosomes and analysis of centromeric DNA organization by in 
      situ hybridization. Alpha-satellite DNA probes specific to chromosomes 1, 2, 3, 4 
      + 9, 5 + 19, 6, 7, 8, 10, 11, 13 + 21, 14 + 22, 15, 16, 17, 18, 20, 22, X and Y 
      were suitable for the accurate identification of human chromosomes in metaphase 
      and interphase cells. Cy3-labeled probes had several advantages: (1) a high level 
      of fluorescence (5-10 times more compared with fluorescein-labeled probes); (2) a 
      low level of fluorescence in solution, allowing the detection of target 
      chromosomes in situ during hybridization without the washing of slides; and (3) 
      high resistance to photobleaching during prolonged (1-2 h) exposure to strong 
      light, thus allowing the use of a high energy mercury lamp or a long integration 
      time during image acquisition in digital imaging microscopy for the determination 
      of weak signals. For di- and multicolor fluorescence in situ hybridization 
      (FISH), we successfully used different combinations of directly fluorophorated 
      probes with preservation of images by conventional microscopy or by digital 
      imaging microscopy. FluorX and Cy3 dyes allowed the use of cosmid probes for 
      mapping in a one-step hybridization experiment. Cyanine-labeled fluorophorated 
      DNA probes offer additional possibilities for rapid chromosome detection during a 
      simple 15-min FISH procedure, and can be recommended for basic research and 
      clinical studies, utilizing FISH.
FAU - Yurov, Y B
AU  - Yurov YB
AD  - National Research Centre of Mental Health, Russian Academy of Medical Sciences, 
      Moscow, Russia.
FAU - Soloviev, I V
AU  - Soloviev IV
FAU - Vorsanova, S G
AU  - Vorsanova SG
FAU - Marcais, B
AU  - Marcais B
FAU - Roizes, G
AU  - Roizes G
FAU - Lewis, R
AU  - Lewis R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Hum Genet
JT  - Human genetics
JID - 7613873
RN  - 0 (Carbocyanines)
RN  - 0 (DNA Probes)
RN  - 0 (Fluoresceins)
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Carbocyanines
MH  - Chromosomes, Human/*genetics
MH  - *DNA Probes
MH  - Fluoresceins
MH  - *Fluorescent Dyes
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Microscopy, Fluorescence
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
AID - 10.1007/BF02185780 [doi]
PST - ppublish
SO  - Hum Genet. 1996 Mar;97(3):390-8. doi: 10.1007/BF02185780.