PMID- 8786106
OWN - NLM
STAT- MEDLINE
DCOM- 19960920
LR  - 20051117
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 32
IP  - 1
DP  - 1996 Feb 15
TI  - The human L35a ribosomal protein (RPL35A) gene is located at chromosome band 
      3q29-qter.
PG  - 148-50
AB  - Using a polymerase chain reaction (PCR)-based strategy that distinguishes 
      functional intron-containing genes from pseudogenes, the chromosomal location of 
      the human intron-containing L35a ribosomal protein gene (rpL35a, HGMW-approved 
      symbol RPL35A) was previously deduced to be onchromosome 18 using DNAs from a 
      panel of human-rodent somatic cell hybrids. The inability to detect rpL35a in a 
      human-hamster somatic cell hybrid containing only human chromosome 18 led to a 
      reinvestigation of the chromosomal location of the human rpL35a gene. A clone 
      containing intron 2 of rpL35a was isolated, sequenced, and used to isolate a P1 
      clone containing the human intron-containing rpL35a gene. By fluorescence in situ 
      hybridization (FISH) analysis with the P1 clone as a probe, the rpL35a gene was 
      located on chromosome band 3q29-qter. This location was confirmed by positive PCR 
      analysis of a human-hamster somatic cell hybrid containing only human chromosome 
      3. The problem of using only human-rodent somatic cell hybrids for chromosome 
      location is discussed.
FAU - Colombo, P
AU  - Colombo P
AD  - Department of Eukaryotic Gene Organisation and Expression, Imperial Cancer 
      Research Fund, Lincoln's Inn Fields, London, United Kingdom.
FAU - Read, M
AU  - Read M
FAU - Fried, M
AU  - Fried M
LA  - eng
SI  - GENBANK/X94619
PT  - Journal Article
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (DNA, Complementary)
RN  - 0 (RPL35A protein, human)
RN  - 0 (Ribosomal Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Banding
MH  - *Chromosome Mapping
MH  - Chromosomes, Human, Pair 3/*genetics/ultrastructure
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - DNA, Complementary/genetics
MH  - Humans
MH  - Hybrid Cells
MH  - In Situ Hybridization, Fluorescence
MH  - Introns
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Pseudogenes
MH  - Ribosomal Proteins/*genetics
EDAT- 1996/02/15 00:00
MHDA- 1996/02/15 00:01
CRDT- 1996/02/15 00:00
PHST- 1996/02/15 00:00 [pubmed]
PHST- 1996/02/15 00:01 [medline]
PHST- 1996/02/15 00:00 [entrez]
AID - S0888-7543(96)90093-2 [pii]
AID - 10.1006/geno.1996.0093 [doi]
PST - ppublish
SO  - Genomics. 1996 Feb 15;32(1):148-50. doi: 10.1006/geno.1996.0093.