PMID- 8786175
OWN - NLM
STAT- MEDLINE
DCOM- 19960920
LR  - 20171206
IS  - 0391-3988 (Print)
IS  - 0391-3988 (Linking)
VI  - 19
IP  - 4
DP  - 1996 Apr
TI  - Effect of ELAD liver support on plasma HGF and TGF-beta 1 in acute liver failure.
PG  - 240-4
AB  - The aim of this study was to investigate the effects of treatment with the 
      extracorporeal liver assist device (ELAD) in patients with acute liver failure 
      (ALF) on plasma hepatocyte growth factor (HGF), the most potent growth factor, 
      and transforming growth factor-beta 1 (TGF-beta 1), an inhibitory factor for 
      liver regeneration. Initial plasma HGF, measured by ELISA, was significantly 
      increased in the ALF patients (7.86 +/- SEM 1.76 ng/ml) compared with normal 
      subjects (0.10 +/- 0.02 ng/ml, p < 0.001). After 6 hours of ELAD haemoperfusion, 
      plasma HGF increased further (30.5 +/- 6.19 ng/ml, p < 0.001), with a subsequent 
      decrease towards the initial value by 48 hours. Initial plasma levels of TGF-beta 
      1 determined by ELISA were significantly increased in the ALF patients (43.4 +/- 
      5.9 ng/ml) compared with normal subjects (25.1 +/- 2.3 ng/ml, p < 0.01), but 
      there was no change in plasma TGF-beta 1 during the study period in either the 
      ELAD or control ALF group. As HGF is a heparin-binding growth factor and similar 
      changes in HGF were observed during CVVHD, one possible explanation is that 
      heparin administered as anticoagulant for extracorporeal circulation is involved 
      in the effects observed on HGF.
FAU - Miwa, Y
AU  - Miwa Y
AD  - Institute of Liver Studies, King's College School of Medicine & Dentistry, 
      London, UK.
FAU - Ellis, A J
AU  - Ellis AJ
FAU - Hughes, R D
AU  - Hughes RD
FAU - Langley, P G
AU  - Langley PG
FAU - Wendon, J A
AU  - Wendon JA
FAU - Williams, R
AU  - Williams R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Artif Organs
JT  - The International journal of artificial organs
JID - 7802649
RN  - 0 (Transforming Growth Factor beta)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Artificial Organs/*standards
MH  - Enzyme-Linked Immunosorbent Assay
MH  - *Extracorporeal Circulation
MH  - Female
MH  - Hemoperfusion
MH  - Heparin/administration & dosage/therapeutic use
MH  - Hepatocyte Growth Factor/*blood
MH  - Humans
MH  - Liver Failure, Acute/*therapy
MH  - Liver Regeneration
MH  - Male
MH  - Middle Aged
MH  - Molecular Weight
MH  - Transforming Growth Factor beta/*blood
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
PST - ppublish
SO  - Int J Artif Organs. 1996 Apr;19(4):240-4.