PMID- 8786403
OWN - NLM
STAT- MEDLINE
DCOM- 19960920
LR  - 20190512
IS  - 0022-3069 (Print)
IS  - 0022-3069 (Linking)
VI  - 55
IP  - 4
DP  - 1996 Apr
TI  - Major histocompatibility complex class I expression on neurons in subacute 
      sclerosing panencephalitis and experimental subacute measles encephalitis.
PG  - 435-43
AB  - Lack of major histocompatibility class I antigens on neurons has been implicated 
      as a possible mechanism for viral persistence in the brain since these antigens 
      are required for cytotoxic T-lymphocyte recognition of infected cells. In 
      subacute sclerosing panencephalitis (SSPE), measles virus (MV) persists in 
      neurons, resulting in a fatal chronic infection. MHC class I mRNA expression was 
      examined in formalin-fixed brain tissue from 6 SSPE patients by in situ 
      hybridization. In addition MHC class I protein expression in MV-infected neurons 
      was examined in experimental Subacute Measles Encephalitis (SME) by double 
      immunohistochemistry. MHC class I mRNA expression was found to be upregulated in 
      SSPE tissues studied, and in 5 out of 6 cases the expression was definitively 
      seen on neurons. The percentage of neurons expressing MHC class I mRNA ranged 
      between 20 to 84% in infected areas. There was no correlation between the degree 
      of infection and expression of MHC class I molecules on neurons. Importantly, the 
      number of neurons co-expressing MHC class I and MV antigens was markedly low, 
      varying between 2 to 8%. Similar results were obtained in SME where 20 to 30% of 
      the neurons expressed MHC class I but <8% co-expressed MHC class I and MV 
      antigens. Perivascular infiltrating cells in the infected regions in SME 
      expressed IFNgamma immunoreactivity. The results suggest that MV may not be 
      directly involved in the induction of MHC class I on neurons and that cytokines 
      such as IFNgamma may play an important role. Furthermore, the paucity of neurons 
      co-expressing MHC class I and MV antigens in SSPE and SME suggests that such 
      cells are either rapidly cleared by cytotoxic T lymphocytes (CTL), or, 
      alternatively, lack of co-expression of MHC class I on MV infected neurons favors 
      MV persistence in these cells by escaping CTL recognition.
FAU - Gogate, N
AU  - Gogate N
AD  - Department of Neurology, University of Maryland at Baltimore, 21201, USA.
FAU - Swoveland, P
AU  - Swoveland P
FAU - Yamabe, T
AU  - Yamabe T
FAU - Verma, L
AU  - Verma L
FAU - Woyciechowska, J
AU  - Woyciechowska J
FAU - Tarnowska-Dziduszko, E
AU  - Tarnowska-Dziduszko E
FAU - Dymecki, J
AU  - Dymecki J
FAU - Dhib-Jalbut, S
AU  - Dhib-Jalbut S
LA  - eng
GR  - P50 NS20022-09A1/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Neuropathol Exp Neurol
JT  - Journal of neuropathology and experimental neurology
JID - 2985192R
SB  - IM
MH  - Adult
MH  - Animals
MH  - Child
MH  - *Encephalitis Viruses
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Male
MH  - Measles/*pathology
MH  - Neurons/*metabolism
MH  - Rats
MH  - Subacute Sclerosing Panencephalitis/*pathology
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - 10.1097/00005072-199604000-00006 [doi]
PST - ppublish
SO  - J Neuropathol Exp Neurol. 1996 Apr;55(4):435-43. doi: 
      10.1097/00005072-199604000-00006.