PMID- 8787770
OWN - NLM
STAT- MEDLINE
DCOM- 19961023
LR  - 20211109
IS  - 0894-1491 (Print)
IS  - 0894-1491 (Linking)
VI  - 16
IP  - 1
DP  - 1996 Jan
TI  - Oligodendrocyte-type-2 astrocyte (O-2A) progenitor cells derived from adult rat 
      spinal cord: in vitro characteristics and response to PDGF, bFGF and NT-3.
PG  - 16-26
AB  - We have analysed in detail the properties of oligodendrocyte-type-2 astrocyte 
      (O-2A) progenitor cells derived from the spinal cords of adult rats to gain 
      further insights into the mechanisms that control the generation of 
      oligodendrocytes in the healthy and demyelinated adult central nervous systems 
      (CNS). When O-2A progenitor cells from adult spinal cord are exposed in vitro to 
      the AA homodimeric form of platelet-derived growth factor (PDGF-AA), they express 
      a unipolar morphology, an O4-positive, vimentin-negative antigenic phenotype, 
      divide at slow rates, and appear to generate oligodendrocytes by asymmetric 
      division and differentiation. Furthermore, exposure of these cells to PDGF-AA is 
      sufficient to stimulate their proliferation at clonal density. When adult spinal 
      cord O-2A progenitor cells are exposed simultaneously to PDGF-AA and basic 
      fibroblast growth factor (PDGF/bFGF), they are almost completely inhibited from 
      differentiating into oligodendrocytes, divide more rapidly than cells treated 
      with PDGF-AA, and express a bipolar morphology and an O4-negative, 
      vimentin-positive antigenic phenotype. These findings indicate that adult spinal 
      cord O-2A progenitor cells resemble in many aspects their well-characterised 
      adult optic nerve counterparts. In addition, evidence is presented to indicate 
      that neurotrophin-3 (NT-3) is not mitogenic for adult spinal cord O-2A progenitor 
      cells and that it does not enhance their proliferative response to PDGF-AA or 
      PDGF/bFGF. Since relatively large numbers of O-2A progenitor cells can be 
      obtained from adult spinal cord, it should facilitate the further 
      characterisation of these cells.
FAU - Engel, U
AU  - Engel U
AD  - Ludwig Institute for Cancer Research, London, England.
FAU - Wolswijk, G
AU  - Wolswijk G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Glia
JT  - Glia
JID - 8806785
RN  - 0 (Growth Substances)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Animals
MH  - Astrocytes/*drug effects
MH  - Bromodeoxyuridine/metabolism
MH  - Cell Differentiation/drug effects
MH  - Cell Division/drug effects
MH  - Cell Polarity/drug effects
MH  - Cells, Cultured
MH  - Female
MH  - Fibroblast Growth Factor 2/pharmacology
MH  - Growth Substances/*pharmacology
MH  - Immunohistochemistry
MH  - Nerve Growth Factors/pharmacology
MH  - Neurotrophin 3
MH  - Oligodendroglia/*drug effects
MH  - Optic Nerve/cytology/drug effects
MH  - Platelet-Derived Growth Factor/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Spinal Cord/*cytology
MH  - Stem Cells/*drug effects
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1002/(SICI)1098-1136(199601)16:1<16::AID-GLIA3>3.0.CO;2-9 [pii]
AID - 10.1002/(SICI)1098-1136(199601)16:1<16::AID-GLIA3>3.0.CO;2-9 [doi]
PST - ppublish
SO  - Glia. 1996 Jan;16(1):16-26. doi: 
      10.1002/(SICI)1098-1136(199601)16:1<16::AID-GLIA3>3.0.CO;2-9.