PMID- 8788206
OWN - NLM
STAT- MEDLINE
DCOM- 19961022
LR  - 20190512
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 54
IP  - 2
DP  - 1996 Feb
TI  - Expression of monocyte chemoattractant protein-1 in the corpus luteum of the rat.
PG  - 513-20
AB  - The known accumulation of macrophages in corpora lutea (CL) at the time of luteal 
      regression prompted us to investigate whether the chemoattractant protein 
      monocyte chemoattractant protein-1 (MCP-1) is expressed in the rat CL. On the day 
      of confirmed mating (Day 0 of pregnancy), regressing CL from the previous 
      (nonfertile) estrous cycle contained immunodetectable MCP-1 and numerous 
      monocytes/macrophages, whereas the newly formed CL of pregnancy, within the same 
      ovary, contained little MCP-1 and few monocytes/macrophages. MCP-1 diminished in 
      the regressing CL on Days 3 and 9 of pregnancy, although numerous 
      monocytes/macrophages remained. The CL of pregnancy on Days 3 and 9 of pregnancy 
      contained minimal MCP-1 and relatively few monocytes/macrophages. By Days 17 and 
      21 of pregnancy, however, prior to parturition and prior to an accumulation of 
      monocytes/macrophages, expression of MCP-1 increased in the CL of pregnancy. 
      Northern blots revealed a resurgence of luteal MCP-1 mRNA on Day 21 of pregnancy: 
      3805 +/- 1077 on Day 21 vs. 1059 +/- 177 on Day 9 (p < 0.05; expressed as 
      densitometric units relative to beta-actin). In conclusion, the expression of 
      MCP-1 in the rat CL in association with, or preceding, the appearance of 
      monocytes/macrophages at the time of luteal regression is consistent with the 
      known role of MCP-1 as a potent chemoattractant for monocytes/macrophages. This 
      suggests that MCP-1 might have a prominent role in the immunological process of 
      luteal regression.
FAU - Townson, D H
AU  - Townson DH
AD  - Department of Physiology, University of Michigan Medical School, Ann Arbor 
      48109-0622, USA. townson@umich.edu
FAU - Warren, J S
AU  - Warren JS
FAU - Flory, C M
AU  - Flory CM
FAU - Naftalin, D M
AU  - Naftalin DM
FAU - Keyes, P L
AU  - Keyes PL
LA  - eng
GR  - P30 HD18258/HD/NICHD NIH HHS/United States
GR  - T32-HD07048/HD/NICHD NIH HHS/United States
GR  - U54 HD29184/HD/NICHD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Chemokine CCL2/analysis/*genetics
MH  - Corpus Luteum/chemistry/cytology/*metabolism
MH  - Female
MH  - *Gene Expression
MH  - Immunohistochemistry
MH  - Luteolysis
MH  - Macrophages
MH  - Molecular Sequence Data
MH  - Monocytes
MH  - Pregnancy
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
AID - 10.1095/biolreprod54.2.513 [doi]
PST - ppublish
SO  - Biol Reprod. 1996 Feb;54(2):513-20. doi: 10.1095/biolreprod54.2.513.