PMID- 8788410
OWN - NLM
STAT- MEDLINE
DCOM- 19970212
LR  - 20190624
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 294
IP  - 1
DP  - 1995 Dec 27
TI  - Nitric oxide donors induce penile erection and yawning when injected in the 
      central nervous system of male rats.
PG  - 1-9
AB  - In order to provide further support for a role of central nitric oxide as a 
      mediator of penile erection and yawning, the nitric oxide donors sodium 
      nitroprusside, hydroxylamine, isoamyl nitrite and 
      S-nitroso-N-acetyl-penicillamine were injected into the lateral ventricles 
      (i.c.v.) or into the paraventricular nucleus of the hypothalamus of male rats. Of 
      the above compounds injected i.c.v., only isoamyl nitrite (10-100 micrograms) 
      induced penile erection and yawning, while the others induced dramatic behavioral 
      changes, such as motor hyperactivity and convulsions, that masked the above 
      responses. Nevertheless, nitric oxide donors in doses ranging from 10 to 50 
      micrograms, for except S-nitroso-N-acetyl-penicillamine that was injected only at 
      the dose of 10 micrograms and isoamyl nitrite that was not injected at all 
      because of poor solubility, induced penile erection and yawning when injected in 
      the paraventricular nucleus. Nitric oxide donor-induced responses were prevented 
      by methylene blue and LY 83583, inhibitors of guanylate cyclase, the best known 
      target of nitric oxide, given i.c.v. but not in the paraventricular nucleus. 
      However, 8-bromo-guanosine 3':5'-cyclic monophosphate (8-Br-cGMP), a stable cGMP 
      analog, and hemoglobin, a nitric oxide scavenger, were ineffective in inducing 
      and preventing, respectively, penile erection and yawning when injected either 
      i.c.v. or in the paraventricular nucleus. Nitric oxide donor-induced responses 
      were also prevented by the nonapeptide oxytocin receptor antagonist 
      d(CH2)5-Tyr(Me)-Orn8-vasotocin given i.c.v. but not in the paraventricular 
      nucleus. The present results suggest that nitric oxide donors induce penile 
      erection and yawning by activating central oxytocinergic transmission in the 
      paraventricular nucleus of the hypothalamus via a cGMP-independent mechanism.
FAU - Melis, M R
AU  - Melis MR
AD  - Bernard B. Brodie Department of Neuroscience, University of Cagliari, Italy.
FAU - Argiolas, A
AU  - Argiolas A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Hemoglobins)
RN  - 0 (Receptors, Oxytocin)
RN  - 31356-94-2 (8-bromocyclic GMP)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 50-56-6 (Oxytocin)
RN  - 77327-45-8 (oxytocin,1-(beta-mercapto-(beta, beta-cyclopentamethylene)propionic 
      acid)-Tyr(OMe)(2)-Orn(8)-)
RN  - EC 4.6.1.2 (Guanylate Cyclase)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - Animals
MH  - Central Nervous System/*physiology
MH  - Cyclic GMP/analogs & derivatives/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Activation/drug effects
MH  - Free Radical Scavengers/pharmacology
MH  - Guanylate Cyclase/antagonists & inhibitors/metabolism
MH  - Hemoglobins/pharmacology
MH  - Injections, Intraventricular
MH  - Male
MH  - Nitric Oxide/metabolism/*physiology
MH  - Oxytocin/analogs & derivatives/pharmacology
MH  - Paraventricular Hypothalamic Nucleus/physiology
MH  - Penile Erection/*drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Oxytocin/antagonists & inhibitors
MH  - Yawning/*drug effects
EDAT- 1995/12/27 00:00
MHDA- 1995/12/27 00:01
CRDT- 1995/12/27 00:00
PHST- 1995/12/27 00:00 [pubmed]
PHST- 1995/12/27 00:01 [medline]
PHST- 1995/12/27 00:00 [entrez]
AID - 0014299995005080 [pii]
AID - 10.1016/0014-2999(95)00508-0 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 1995 Dec 27;294(1):1-9. doi: 10.1016/0014-2999(95)00508-0.