PMID- 8788944
OWN - NLM
STAT- MEDLINE
DCOM- 19961024
LR  - 20190512
IS  - 0022-3751 (Print)
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 489 ( Pt 3)
IP  - Pt 3
DP  - 1995 Dec 15
TI  - P2 purinoceptor-mediated depolarization of rat supraoptic neurosecretory cells in 
      vitro.
PG  - 805-11
AB  - 1. Intracellular recordings were obtained from supraoptic magnocellular 
      neurosecretory cells (MNCs) in superfused explants of rat hypothalamus. 
      Application of ATP and UTP, but not adenosine, produced TTX-insensitive 
      depolarizations accompanied by increases of input conductance. 2. The P2X 
      agonists alpha,beta-methylene ATP, beta,gamma-methylene ATP and 2-methylthio ATP 
      mimicked the effects of ATP in > 77% of the cells tested. Depolarizing responses 
      to ATP were reversibly inhibited by PPADS (pyridoxal-phosphate-6-azophenyl-2',4'- 
      disulphonic acid; IC50 approximately 0.5 microM), a selective P2X antagonist. 3. 
      The reversal potential of responses to ATP (-37 mV) was not strongly affected by 
      intracellular Cl- injection or by removal of Cl- from the external solution. The 
      reversal potential of responses to the most potent P2X agonist, 
      alpha,beta-methylene ATP, was -29 mV. These values suggest the involvement of 
      non-selective cationic channels, a finding which is consistent with the 
      ionotropic cationic channel structure of cloned P2X purinoceptors. 4. The 
      reversal potential of UTP-mediated responses (-33 mV) was also consistent with 
      the involvement of non-selective cationic channels. Since cloned P2U receptors 
      display homology with G-protein-coupled receptors, cationic channels modulated by 
      UTP are probably different from those mediating P2X responses.
FAU - Hiruma, H
AU  - Hiruma H
AD  - Centre for Research in Neuroscience, McGill University, Montreal, Canada.
FAU - Bourque, C W
AU  - Bourque CW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Adenine Nucleotides)
RN  - 0 (Ligands)
RN  - 0 (Platelet Aggregation Inhibitors)
RN  - 0 (Purinergic P2 Receptor Agonists)
RN  - 0 (Purinergic P2 Receptor Antagonists)
RN  - 0 (Receptors, Purinergic P2)
RN  - 149017-66-3 (pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid)
RN  - 5V5IOJ8338 (Pyridoxal Phosphate)
RN  - UT0S826Z60 (Uridine Triphosphate)
SB  - IM
MH  - Adenine Nucleotides/antagonists & inhibitors/pharmacology
MH  - Animals
MH  - Electrophysiology
MH  - In Vitro Techniques
MH  - Ligands
MH  - Male
MH  - Membrane Potentials/drug effects
MH  - Microelectrodes
MH  - Neurons/drug effects/physiology
MH  - Neurosecretory Systems/cytology/*physiology
MH  - Patch-Clamp Techniques
MH  - Platelet Aggregation Inhibitors/pharmacology
MH  - Purinergic P2 Receptor Agonists
MH  - Purinergic P2 Receptor Antagonists
MH  - Pyridoxal Phosphate/analogs & derivatives/pharmacology
MH  - Rats
MH  - Receptors, Purinergic P2/*physiology
MH  - Supraoptic Nucleus/cytology/*physiology
MH  - Uridine Triphosphate/pharmacology
PMC - PMC1156849
EDAT- 1995/12/15 00:00
MHDA- 1995/12/15 00:01
PMCR- 1995/12/15
CRDT- 1995/12/15 00:00
PHST- 1995/12/15 00:00 [pubmed]
PHST- 1995/12/15 00:01 [medline]
PHST- 1995/12/15 00:00 [entrez]
PHST- 1995/12/15 00:00 [pmc-release]
AID - 10.1113/jphysiol.1995.sp021093 [doi]
PST - ppublish
SO  - J Physiol. 1995 Dec 15;489 ( Pt 3)(Pt 3):805-11. doi: 
      10.1113/jphysiol.1995.sp021093.