PMID- 8790406
OWN - NLM
STAT- MEDLINE
DCOM- 19961024
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 93
IP  - 18
DP  - 1996 Sep 3
TI  - FHIT gene alterations in head and neck squamous cell carcinomas.
PG  - 9770-5
AB  - To determine whether the FHIT gene at 3p14.2 is altered in head and neck squamous 
      cell carcinomas (HNSCC), we examined 26 HNSCC cell lines for deletions within the 
      FHIT locus by Southern analysis, for allelic losses of specific exons FHIT by 
      fluorescence in situ hybridization (FISH) and for integrity of FHIT transcripts. 
      Three cell lines exhibited homozygous deletions within the FHIT gene, 55% (15/25) 
      showed the presence of aberrant transcripts, and 65% (13/20) showed the presence 
      of multiple cell populations with losses of different portions of FHIT alleles by 
      FISH of FHIT genomic clones to interphase nuclei. When the data obtained by FISH 
      and by reverse transcriptase-PCR analyses are combined, 22 of 26 cell lines 
      showed alterations of at least one allele of the FHIT gene. Our data indicate 
      that the FHIT gene is disrupted in HNSCCs and hence, loss of FHIT function may be 
      important in the development and/or progression of head and neck cancers.
FAU - Virgilio, L
AU  - Virgilio L
AD  - Kimmel Cancer Institute, Jefferson Medical College, Philadelphia, PA 19107, USA.
FAU - Shuster, M
AU  - Shuster M
FAU - Gollin, S M
AU  - Gollin SM
FAU - Veronese, M L
AU  - Veronese ML
FAU - Ohta, M
AU  - Ohta M
FAU - Huebner, K
AU  - Huebner K
FAU - Croce, C M
AU  - Croce CM
LA  - eng
GR  - CA21124/CA/NCI NIH HHS/United States
GR  - CA39860/CA/NCI NIH HHS/United States
GR  - DE10513/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proteins)
RN  - 0 (fragile histidine triad protein)
RN  - EC 3.6.- (Acid Anhydride Hydrolases)
SB  - IM
MH  - *Acid Anhydride Hydrolases
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Cloning, Molecular
MH  - Head and Neck Neoplasms/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Neoplasm Proteins/*genetics
MH  - Proteins/*genetics
MH  - Transcription, Genetic
MH  - Tumor Cells, Cultured
PMC - PMC38504
EDAT- 1996/09/03 00:00
MHDA- 1996/09/03 00:01
PMCR- 1997/03/03
CRDT- 1996/09/03 00:00
PHST- 1996/09/03 00:00 [pubmed]
PHST- 1996/09/03 00:01 [medline]
PHST- 1996/09/03 00:00 [entrez]
PHST- 1997/03/03 00:00 [pmc-release]
AID - 10.1073/pnas.93.18.9770 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9770-5. doi: 10.1073/pnas.93.18.9770.