PMID- 8790742
OWN - NLM
STAT- MEDLINE
DCOM- 19961204
LR  - 20191210
IS  - 0001-6489 (Print)
IS  - 0001-6489 (Linking)
VI  - 116
IP  - 3
DP  - 1996 May
TI  - Responsiveness of alpha 1 and beta 1 cochlear Na, K-ATPase isoforms to thyroid 
      hormone.
PG  - 422-8
AB  - The effects of thyroid hormone on Na, K-ATPase subunit isoforms under euthyroid 
      (EUTH), hypothyroid (HYPO) and hyperthyroid (HYPER) states were investigated via 
      immunocytochemistry and the use of polyclonal antibodies specific to each isoform 
      (alpha 1, alpha 2, alpha 3 and beta 1, beta 2). In HYPO animals, there was a 
      distinct decrease in Na, K-ATPase alpha 1 isoform immunoreactivity in the stria 
      vascularis (SV), spiral ganglion (SG) cells, spiral limbus (SLi) and cochlear 
      nerve (CN) as compared with that in EUTH animals by the 17th day of the 
      experiment. Immunostaining of the alpha 1 isoform increased in HYPER animals as 
      compared with that in HYPO animals, and reached a level comparable to that in 
      EUTH animals after 2 days of triiodothyronine (T3) treatment. Levels of alpha 2, 
      alpha 3 and beta 2 isoforms did not appear to be affected by T3 administration. 
      By the 19th day of a low I2 diet, the immunoreactive intensity of the beta 1 
      isoform was reduced in cochlear tissues of HYPO animals as compared with that in 
      EUTH animals. The immunoreactivity of the beta 1 isoform increased after 
      treatment with T3 for 4 days and was comparable with levels in EUTH animals. 
      These data indicate that alpha 1 and beta 1 isoforms within specific cochlear 
      regions of the adult rat are responsive to thyroid hormone.
FAU - Zuo, J
AU  - Zuo J
AD  - Department of Anatomy & Cell Biology, College of Medicine, University of Florida, 
      Gainesville 32610, USA.
FAU - Rarey, K E
AU  - Rarey KE
LA  - eng
GR  - DC00426/DC/NIDCD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Acta Otolaryngol
JT  - Acta oto-laryngologica
JID - 0370354
RN  - 0 (Thyroid Hormones)
RN  - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
SB  - IM
MH  - Animals
MH  - Cochlear Nerve/metabolism
MH  - Culture Techniques
MH  - Immunohistochemistry
MH  - Male
MH  - Organ of Corti/*metabolism
MH  - Rats
MH  - Sodium-Potassium-Exchanging ATPase/*metabolism
MH  - Spiral Ganglion/metabolism
MH  - Thyroid Hormones/*metabolism
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - 10.3109/00016489609137867 [doi]
PST - ppublish
SO  - Acta Otolaryngol. 1996 May;116(3):422-8. doi: 10.3109/00016489609137867.