PMID- 8791251
OWN - NLM
STAT- MEDLINE
DCOM- 19961203
LR  - 20190724
IS  - 0022-510X (Print)
IS  - 0022-510X (Linking)
VI  - 138
IP  - 1-2
DP  - 1996 Jun
TI  - AIDS-associated vacuolar myelopathy and tumor necrosis factor-alpha (TNF alpha).
PG  - 134-44
AB  - The spinal cords from 15 patients with AIDS-associated vacuolar myelopathy (VM), 
      4 AIDS patients without VM, and 5 HIV-seronegative controls, were studied with 
      immunocytochemistry for TNF alpha. CSF and blood from HIV-seropositive patients 
      with VM (n = 16), non-vacuolar myelopathies (n = 8), CNS infection but no 
      clinical myelopathy (n = 31), no clinical or radiological evidence of CNS disease 
      (n = 9), and from 7 HIV-seronegative controls with motor neurone disease were 
      assayed for TNF alpha using an ELISA technique. TNF alpha was present on 
      immunostaining in all the 15 cords with VM studied. The stained cells were 
      macrophages, microglia and endothelial cells. The amount of immunostaining was 
      higher in cords with VM compared with cords from HIV-seropositive patients 
      without VM (p = 0.001). The distribution of staining corresponded to the areas of 
      pathology but did not correlate with the severity of the VM. Immunostaining was 
      also higher in the HIV-seropositive group compared to the HIV-seronegative 
      controls (p = 0.001). There was no significant difference in the levels of TNF 
      alpha in the CSF of patients with VM compared to any of the other groups studied. 
      Blood levels of TNF alpha were lower in the HIV-seropositive controls without CNS 
      disease and in the HIV-seronegative MND controls, than in patients with VM, 
      non-vacuolar myelopathies, and CNS disease. CSF TNF alpha levels did not appear 
      to be a reliable indicator of intramedullary levels. The findings support the 
      hypothesis that TNF alpha may be relevant in the pathogenesis of vacuolar change 
      in VM.
FAU - Tan, S V
AU  - Tan SV
AD  - Department of Neurology, Charing Cross Hospital, London, UK.
FAU - Guiloff, R J
AU  - Guiloff RJ
FAU - Henderson, D C
AU  - Henderson DC
FAU - Gazzard, B G
AU  - Gazzard BG
FAU - Miller, R
AU  - Miller R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Neurol Sci
JT  - Journal of the neurological sciences
JID - 0375403
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Acquired Immunodeficiency Syndrome/*complications/metabolism/pathology
MH  - Adult
MH  - Case-Control Studies
MH  - Enzyme-Linked Immunosorbent Assay
MH  - HIV Seronegativity/physiology
MH  - HIV Seropositivity/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Middle Aged
MH  - Motor Neuron Disease/immunology
MH  - Myelin Sheath/*pathology
MH  - Paraparesis, Tropical Spastic/*complications/metabolism/pathology
MH  - Spinal Cord Diseases/*complications/metabolism/pathology
MH  - Tumor Necrosis Factor-alpha/*analysis/cerebrospinal fluid
MH  - Vacuoles/*pathology
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
AID - 0022-510X(95)00354-5 [pii]
AID - 10.1016/0022-510x(95)00354-5 [doi]
PST - ppublish
SO  - J Neurol Sci. 1996 Jun;138(1-2):134-44. doi: 10.1016/0022-510x(95)00354-5.