PMID- 8792563
OWN - NLM
STAT- MEDLINE
DCOM- 19970203
LR  - 20190830
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 51
IP  - 3-4
DP  - 1996 Jun 1
TI  - Production and characterisation of monoclonal antibodies recognising ovine IgE.
PG  - 235-51
AB  - Two monoclonal antibodies (mAbs), XB6 and YD3, which recognise ovine 
      immunoglobulin E (IgE) were produced. Mast cells isolated from ovine intestinal 
      mucosa were used as a source of IgE to immunize mice. Culture supernatants of 
      hybridomas were screened by immunoassays on small-intestine tissue sections, 
      isolated mucosal cells, and dot blots of lysed mast cell homogenate. Two mAbs 
      were chosen for their specific binding to mast cells. Antigen bound by these mAbs 
      was purified by immunoaffinity chromatography using XB6 mAb, and this produced 
      two bands consistent with IgE heavy chain (86,000 Daltons) and immunoglobulin 
      light chain (28,000 Daltons) when run under reducing conditions on SDS-PAGE gels. 
      Purified IgE was shown on dot blots to react weakly with mAb to chimeric ovine 
      IgE and strongly to polyclonal anti-sheep antibodies. The two mAbs induced an 
      immediate hypersensitivity-like reaction when injected into the skin of sheep. 
      The mAbs bound to mast cells and other mononuclear cells, presumably 
      IgE-secreting B-cells in mesenteric lymph node sections. These mAbs proved useful 
      for detecting IgE-bearing cells in various ovine tissues, for purifying mast 
      cells from cell isolates by panning and immunomagnetic bead separation, for 
      purifying serum IgE using immunoaffinity chromatography and for detecting IgE in 
      an ELISA. Competitive binding assays showed that the two mAbs bind to different 
      epitopes on IgE. These mAbs will be useful in research applications and in 
      diagnostic assays.
FAU - Shaw, R J
AU  - Shaw RJ
AD  - AgResearch, Wallaceville Animal Research Centre, Upper Hutt, New Zealand.
FAU - Grimmett, D J
AU  - Grimmett DJ
FAU - Donaghy, M J
AU  - Donaghy MJ
FAU - Gatehouse, T K
AU  - Gatehouse TK
FAU - Shirer, C L
AU  - Shirer CL
FAU - Douch, P G
AU  - Douch PG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (anti-IgE antibodies)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Anaphylaxis/immunology
MH  - Animals
MH  - Antibodies, Anti-Idiotypic/*biosynthesis/chemistry
MH  - Antibodies, Monoclonal/*biosynthesis/chemistry
MH  - Binding, Competitive/immunology
MH  - Cell Separation/veterinary
MH  - Chromatography, Affinity/veterinary
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Immunoglobulin E/*immunology/isolation & purification
MH  - Immunohistochemistry
MH  - Immunomagnetic Separation/veterinary
MH  - Mast Cells/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Sheep/*immunology
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
AID - 0165-2427(95)05530-4 [pii]
AID - 10.1016/0165-2427(95)05530-4 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 1996 Jun 1;51(3-4):235-51. doi: 
      10.1016/0165-2427(95)05530-4.