PMID- 8792847
OWN - NLM
STAT- MEDLINE
DCOM- 19961022
LR  - 20190819
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 112
IP  - 1
DP  - 1996 Aug 1
TI  - Teratogenic effects of the demethylating agent 5-aza-2'-deoxycytidine in the 
      Swiss Webster mouse.
PG  - 37-43
AB  - 5-Aza-2'-deoxycytidine (d-AZA) replaces cytidine in DNA thereby altering gene 
      expression by passively removing methyl groups. This study determined the 
      temporal patterns of morphological defects induced by d-AZA in mice. The dosages 
      (0, 0.3, or 1.0 mg/kg) were administered by a single i.p. injection on 
      gestational days (GD) 8, 9, 10, or 11. Mice were killed on GD 17 and fetal 
      skeletons examined. The 1.0 mg/kg dose elicited characteristic defects for each 
      treatment day: GD 8, supernumerary ribs, (significantly above background), fused 
      vertebrae and ribs; GD 9, cleft palate and vertebral variations; GD 10, hind limb 
      defects (especially phocomelia); GD 11, digital defects of fore and hindlimbs. 
      The known demethylating ability of d-AZA coupled with the induction of longbone 
      defects only in the hindlimbs suggests that d-AZA may act by disrupting specific 
      hindlimb gene function through DNA hypomethylation.
FAU - Branch, S
AU  - Branch S
AD  - North Carolina State University, Department of Toxicology, Raleigh 27695, USA.
FAU - Francis, B M
AU  - Francis BM
FAU - Brownie, C F
AU  - Brownie CF
FAU - Chernoff, N
AU  - Chernoff N
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Teratogens)
RN  - 776B62CQ27 (Decitabine)
RN  - EC 2.1.1.- (DNA Modification Methylases)
RN  - M801H13NRU (Azacitidine)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Azacitidine/administration & dosage/*analogs & derivatives/toxicity
MH  - Cleft Palate/chemically induced/embryology
MH  - DNA Damage/genetics
MH  - DNA Modification Methylases/*antagonists & inhibitors
MH  - Decitabine
MH  - Dose-Response Relationship, Drug
MH  - Embryonic and Fetal Development/*drug effects
MH  - Enzyme Inhibitors/administration & dosage/*toxicity
MH  - Female
MH  - Gene Expression Regulation, Developmental/drug effects/genetics
MH  - Gestational Age
MH  - Hindlimb/drug effects/embryology
MH  - Injections, Intraperitoneal
MH  - Mice
MH  - Pregnancy
MH  - Ribs/drug effects/embryology
MH  - Spine/drug effects/embryology
MH  - Teratogens/*toxicity
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
AID - 0300483X96881832 [pii]
AID - 10.1016/0300-483x(96)88183-2 [doi]
PST - ppublish
SO  - Toxicology. 1996 Aug 1;112(1):37-43. doi: 10.1016/0300-483x(96)88183-2.