PMID- 8793177
OWN - NLM
STAT- MEDLINE
DCOM- 19961205
LR  - 20191210
IS  - 0143-4160 (Print)
IS  - 0143-4160 (Linking)
VI  - 19
IP  - 5
DP  - 1996 May
TI  - Biochemical and functional heterogeneity of rat cerebrum microsomal membranes in 
      relation to SERCA Ca(2+)-ATPases and Ca2+ release channels.
PG  - 375-81
AB  - Rat cerebrum microsomes were subfractionated on isopycnic linear sucrose (20-42%) 
      density gradients. The Ca2+ loading/release properties and the distribution of 
      intracellular Ca2+ store channels, inositol 1,4,5-trisphosphate (IP3) receptor 
      and ryanodine (Ry) receptor, and SERCA pumps, were monitored in each subfraction 
      by ligand binding and 45Ca2+ loading/release assays. Three different classes of 
      vesicles were identified: (i) heavy density vesicles with high content of Ry 
      receptors and Ca2+ pumps and high thapsigargin (TG)-sensitivity of Ca2+ loading; 
      (ii) intermediate sucrose density vesicles with high content of IP3 receptor, 
      high IP(S)3-sensitivity of Ca2+ loading and low content of Ry receptors; and 
      (iii) light sucrose density vesicles with high content of Ry receptors, low 
      content of IP3 receptors and low content of SERCA pumps highly sensitive to TG. 
      Isolation of molecularly heterogeneous rat cerebrum microsomes and identification 
      of specific Ca2+ loading/release properties support the presence of multiple, 
      potentially active, heterogeneous rapidly exchanging Ca2+ stores in rat cerebrum.
FAU - Nori, A
AU  - Nori A
AD  - Dipartimento di Scienze Biomediche Sperimentali dell'Universita di Padova, Italy.
FAU - Fulceri, R
AU  - Fulceri R
FAU - Gamberucci, A
AU  - Gamberucci A
FAU - Benedetti, A
AU  - Benedetti A
FAU - Volpe, P
AU  - Volpe P
LA  - eng
GR  - NS-29640-03/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Cell Calcium
JT  - Cell calcium
JID - 8006226
RN  - 0 (Calcium Channels)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Brain/*metabolism/ultrastructure
MH  - Calcium/*metabolism
MH  - Calcium Channels/*metabolism
MH  - Calcium-Transporting ATPases/*metabolism
MH  - Centrifugation, Density Gradient
MH  - Ion Transport
MH  - Microsomes/*metabolism
MH  - Rats
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - S0143-4160(96)90110-4 [pii]
AID - 10.1016/s0143-4160(96)90110-4 [doi]
PST - ppublish
SO  - Cell Calcium. 1996 May;19(5):375-81. doi: 10.1016/s0143-4160(96)90110-4.