PMID- 8793382
OWN - NLM
STAT- MEDLINE
DCOM- 19970225
LR  - 20131121
IS  - 0171-9335 (Print)
IS  - 0171-9335 (Linking)
VI  - 70
IP  - 2
DP  - 1996 Jun
TI  - Proliferating and differentiating Schwann cell cultures from embryonic chick 
      sciatic nerve maintained for months in vitro without antimitotics or growth 
      factors.
PG  - 106-16
AB  - In general, the available methods for culturing Schwann cells require specific 
      antibodies and/or the addition of antimitotics to suppress fibroblasts, plus 
      various factors to support their growth. Moreover, the maximal culture period of 
      Schwann cells normally is limited to a few weeks. Here, three easy novel methods 
      to culture Schwann cells from embryonic chick sciatic nerve are presented, that 
      require no growth factors or agents elevating intracellular cAMP. In contrast to 
      the conventional antimitotic treatment with cytosine arabinoside, we use D-valine 
      to suppress fibroblasts. Our modified medium C leads within a few days to highly 
      enriched Schwann cell cultures (culture I). Passage into a serum-reduced medium D 
      allows for differentiating longterm cultures (culture II). In cultures I and II, 
      the rate of cell division is low. However, after passage into serum-containing 
      SC-medium, proliferation increases within one week to high levels (culture III). 
      Cultures II and III can be grown for several months, during which time 
      spontaneous immortalization can occur. The high purity of the cultures of about 
      95% is assessed using glia-specific antibodies for S-100 antigen, HNK-1 epitope, 
      glia fibrillary acidic protein (GFAP), galactocerebroside (Gal C) and 3A7. These 
      culture procedures are easy to perform and are suitable for differentiation, 
      proliferation and coculturing experiments.
FAU - Weiss, B
AU  - Weiss B
AD  - Max Planck Institute for Developmental Biology, Tubingen/Germany.
FAU - Del Turco, D
AU  - Del Turco D
FAU - Layer, P G
AU  - Layer PG
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Eur J Cell Biol
JT  - European journal of cell biology
JID - 7906240
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Culture Media)
RN  - 0 (Growth Substances)
RN  - HG18B9YRS7 (Valine)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/pharmacology
MH  - Cell Culture Techniques/*methods
MH  - Cell Differentiation/physiology
MH  - Cell Division/physiology
MH  - Chick Embryo
MH  - Culture Media
MH  - Fibroblasts/drug effects
MH  - Growth Substances/pharmacology
MH  - Schwann Cells/*cytology
MH  - Sciatic Nerve/*cytology
MH  - Valine/pharmacology
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
PST - ppublish
SO  - Eur J Cell Biol. 1996 Jun;70(2):106-16.