PMID- 8793799
OWN - NLM
STAT- MEDLINE
DCOM- 19970124
LR  - 20210518
IS  - 1046-6673 (Print)
IS  - 1046-6673 (Linking)
VI  - 7
IP  - 6
DP  - 1996 Jun
TI  - Prostaglandin E1 reduces the glomerular mRNA expression of 
      monocyte-chemoattractant protein 1 in anti-thymocyte antibody-induced glomerular 
      injury.
PG  - 897-905
AB  - To study whether prostaglandins (PG) can regulate the mRNA expression of 
      monocyte-chemoattractant protein 1 (MCP-1) in glomerular immune injury, MCP-1 
      mRNA levels were evaluated in anti-thymocyte antibody (ATS) -induced glomerular 
      injury by Northern blotting and reverse transcription-polymerase chain reaction. 
      Immune injury was induced in vivo by the intravenous application of ATS to male 
      Wistar rats and in vitro by the perfusion of isolated rat kidneys with ATS and 
      rat serum. In vivo 3 h and 5 days after antibody application, glomerular mRNA 
      expression of MCP-1 was markedly enhanced compared with controls. In the isolated 
      perfused kidney, antibody and complement also induced an increase in MCP-1 
      expression at 10 min and 60 min after antibody perfusion. When the rats were 
      treated with PGE (250 micrograms, twice daily), the increase in MCP-1 expression 
      was reduced. This was associated with a reduction of intraglomerular recruitment 
      of monocytes/macrophages. In the isolated perfused kidneys, PGE1 (1 mg/L) 
      prevented the antibody- and rat serum-stimulated increase in glomerular MCP-1 
      mRNA expression. These data demonstrate that PGE1 reduces glomerular MCP-1 mRNA 
      expression in glomerulonephritis and in the isolated perfused rat kidney after 
      induction of immune injury with antibody and complement. The data suggest that 
      prostaglandins might mediate MCP-1 effects in glomerular immune injuries.
FAU - Jocks, T
AU  - Jocks T
AD  - Department of Medicine, University of Hamburg, Germany.
FAU - Zahner, G
AU  - Zahner G
FAU - Freudenberg, J
AU  - Freudenberg J
FAU - Wolf, G
AU  - Wolf G
FAU - Thaiss, F
AU  - Thaiss F
FAU - Helmchen, U
AU  - Helmchen U
FAU - Stahl, R A
AU  - Stahl RA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Am Soc Nephrol
JT  - Journal of the American Society of Nephrology : JASN
JID - 9013836
RN  - 0 (Chemokine CCL2)
RN  - 0 (Isoantibodies)
RN  - 0 (RNA, Messenger)
RN  - 0 (anti-Thy antibody)
RN  - F5TD010360 (Alprostadil)
SB  - IM
MH  - Alprostadil/*pharmacology
MH  - Animals
MH  - Blotting, Northern
MH  - Chemokine CCL2/*genetics
MH  - Complement Hemolytic Activity Assay
MH  - Glomerulonephritis/*immunology/*metabolism/pathology
MH  - In Vitro Techniques
MH  - Isoantibodies/*immunology
MH  - Kidney Glomerulus/*metabolism/pathology
MH  - Male
MH  - Perfusion
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Transcription, Genetic
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
AID - 10.1681/ASN.V76897 [doi]
PST - ppublish
SO  - J Am Soc Nephrol. 1996 Jun;7(6):897-905. doi: 10.1681/ASN.V76897.