PMID- 8794855
OWN - NLM
STAT- MEDLINE
DCOM- 19961024
LR  - 20240104
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 134
IP  - 5
DP  - 1996 Sep
TI  - Centromere and telomere movements during early meiotic prophase of mouse and man 
      are associated with the onset of chromosome pairing.
PG  - 1109-25
AB  - The preconditions and early steps of meiotic chromosome pairing were studied by 
      fluorescence in situ hybridization (FISH) with chromosome-specific DNA probes to 
      mouse and human testis tissue sections. Premeiotic pairing of homologous 
      chromosomes was not detected in spermatogonia of the two species. FISH with 
      centromere- and telomere-specific DNA probes in combination with immunostaining 
      (IS) of synaptonemal complex (SC) proteins to testis sections of prepuberal mice 
      at days 4-12 post partum was performed to study sequentially the meiotic pairing 
      process. Movements of centromeres and then telomeres to the nuclear envelope, and 
      of telomeres along the nuclear envelope leading to the formation of a chromosomal 
      bouquet were detected during mouse prophase. At the bouquet stage, pairing of a 
      mouse chromosome-8-specific probe was observed. SC-IS and simultaneous telomere 
      FISH revealed that axial element proteins appear as large aggregates in mouse 
      meiocytes when telomeres are attached to the nuclear envelope. Axial element 
      formation initiates during tight telomere clustering and transverse filament-IS 
      indicated the initiation of synapsis during this stage. Comparison of telomere 
      and centromere distribution patterns of mouse and human meiocytes revealed 
      movements of centromeres and then telomeres to the nuclear envelope and 
      subsequent bouquet formation as conserved motifs of the pairing process. 
      Chromosome painting in human spermatogonia revealed compacted, largely mutually 
      exclusive chromosome territories. The territories developed into long, thin 
      threads at the onset of meiotic prophase. Based on these results a unified model 
      of the pairing process is proposed.
FAU - Scherthan, H
AU  - Scherthan H
AD  - Department of Human Genetics and Human Biology, University of Kaiserslautern, 
      Germany.
FAU - Weich, S
AU  - Weich S
FAU - Schwegler, H
AU  - Schwegler H
FAU - Heyting, C
AU  - Heyting C
FAU - Harle, M
AU  - Harle M
FAU - Cremer, T
AU  - Cremer T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
SB  - IM
MH  - Animals
MH  - Cell Nucleus/physiology
MH  - Centromere/*physiology
MH  - Chromosomes/physiology
MH  - Chromosomes, Human, Pair 1/physiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Meiosis/*physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Movement
MH  - Nuclear Envelope/physiology
MH  - Prophase/*physiology
MH  - Spermatogonia/physiology
MH  - Staining and Labeling
MH  - Synaptonemal Complex/physiology
MH  - Telomere/*physiology
MH  - Testis/cytology/physiology
PMC - PMC2120985
EDAT- 1996/09/01 00:00
MHDA- 1996/09/01 00:01
PMCR- 1997/03/01
CRDT- 1996/09/01 00:00
PHST- 1996/09/01 00:00 [pubmed]
PHST- 1996/09/01 00:01 [medline]
PHST- 1996/09/01 00:00 [entrez]
PHST- 1997/03/01 00:00 [pmc-release]
AID - 96387320 [pii]
AID - 10.1083/jcb.134.5.1109 [doi]
PST - ppublish
SO  - J Cell Biol. 1996 Sep;134(5):1109-25. doi: 10.1083/jcb.134.5.1109.