PMID- 8795271
OWN - NLM
STAT- MEDLINE
DCOM- 19961231
LR  - 20190706
IS  - 0009-2363 (Print)
IS  - 0009-2363 (Linking)
VI  - 44
IP  - 8
DP  - 1996 Aug
TI  - HPLC determination of carnitine and acylcarnitines in human plasma by means of 
      fluorescence labeling using 2-(4-hydrazinocarbonylphenyl)-4,5-diphenylimidazole.
PG  - 1525-9
AB  - Carnitine and acylcarnitines are important substances involved in the oxidation 
      and metabolism of fatty acids. An HPLC method is presented for the quantitative 
      analysis of these compounds. The method is based on the detection of fluorescent 
      derivatives of carnitine and short- and medium-chain acylcarnitines labeled with 
      2-(4-hydrazinocarbonylphenyl)-4,5-diphenylimidazole (HCPI). The labeling of 
      carnitine and acylcarnitines with HCPI was performed at room temperature for 1 h 
      using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide as a condensing reagent. The 
      analytes prepared using cation-exchange cartridges were separated on an 
      octadecylsilyl silica gel (ODS) column by a gradient elution system of 
      acetonitrile/Tris-HCI buffer and determined using a synthetic internal standard. 
      Fluorescence detection was performed at 475 nm with excitation at 340 nm. The 
      detection limits for carnitine, acetyl-, propionyl-, hexanoyl- and 
      octanoylcarnitine ranged from 0.24 to 1.97 nmol per ml human plasma, at a 
      signal-to-noise ratio of 3. The within-day and between-day precision of the assay 
      for carnitine and acylcarnitines in plasma samples had relative standard 
      deviations (RSDs) lower than 10.3%. The concentration of free carnitine, 
      acylcarnitines and total carnitine in human plasma could be successfully 
      determined by the proposed method.
FAU - Kuroda, N
AU  - Kuroda N
AD  - School of Pharmaceutical Sciences, Nagasaki University, Japan.
FAU - Ohyama, Y
AU  - Ohyama Y
FAU - Nakashima, K
AU  - Nakashima K
FAU - Nakashima, K
AU  - Nakashima K
FAU - Akiyama, S
AU  - Akiyama S
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Chem Pharm Bull (Tokyo)
JT  - Chemical & pharmaceutical bulletin
JID - 0377775
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Hydrazines)
RN  - 0 (Imidazoles)
RN  - 151589-38-7 (2-(4-hydrazinocarbonylphenyl)-4,5-diphenylimidazole)
RN  - S7UI8SM58A (Carnitine)
SB  - IM
MH  - Adult
MH  - Carnitine/*analogs & derivatives/*blood
MH  - Chromatography, High Pressure Liquid
MH  - Female
MH  - Fluorescent Dyes
MH  - Humans
MH  - Hydrazines
MH  - Imidazoles
MH  - Male
MH  - Middle Aged
MH  - Spectrometry, Fluorescence
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
AID - 10.1248/cpb.44.1525 [doi]
PST - ppublish
SO  - Chem Pharm Bull (Tokyo). 1996 Aug;44(8):1525-9. doi: 10.1248/cpb.44.1525.