PMID- 8795457
OWN - NLM
STAT- MEDLINE
DCOM- 19961010
LR  - 20220331
IS  - 0014-4835 (Print)
IS  - 0014-4835 (Linking)
VI  - 62
IP  - 4
DP  - 1996 Apr
TI  - Transforming growth factor-beta 1 induces alpha-smooth muscle-actin expression in 
      cultured human and monkey trabecular meshwork.
PG  - 389-97
AB  - Transforming growth factor-beta 1 (TGF-beta 1) induces alpha-smooth muscle 
      (sm)-actin expression and a contractile myofibroblast-like phenotype in a 
      considerable number of different cell types. Since alpha-sm-actin is expressed in 
      some of the resident human trabecular meshwork (TM) cells in situ, and TGF-beta 1 
      is synthesized by TM in vitro, alpha-sm-actin expression in TM might also be 
      under the influence of TGF-beta 1. To assess this question, TM cultures were 
      initiated from the eyes of three human donors and three cynomolgus monkeys. 
      Various doses of TGF-beta 1 (0.5-5 ng ml-1) were added to confluent cultures from 
      third to fourth passages. Experiments were performed in the presence of fetal 
      calf serum or under chemically defined serum-free conditions. Four days after 
      treatment, cells were stained immunocytochemically for alpha-sm-actin, and the 
      number of positively labelled cells was quantitatively evaluated. In addition, 
      reverse transcription polymerase chain reaction (PCR) was performed using 
      oligonucleotide primers specific for alpha-sm-actin. In control cultures 
      supplemented with serum, 19.0 +/- 9.4% of human meshwork cells, and 10.2 +/- 4.5% 
      of monkey meshwork cells expressed immunoreactivity for alpha-sm-actin. In human 
      cultures, this number was significantly higher in serum-free conditions (34.1 +/- 
      7.7%). Treatment with TGF-beta 1 induced alpha-sm-actin expression in a 
      dose-dependent manner. At 5 ng ml-1 TGF-beta 1, 75.5 +/- 7.1% of human meshwork 
      cells expressed distinct stress fibers that stained positively for alpha-sm-actin 
      (P < or = 0.01). A similar albeit smaller increase in alpha-sm-actin positive 
      cells was observed in monkey cultures following treatment with TGF-beta 1. These 
      effects were seen with and without serum, but not when TGF-beta 1 was 
      supplemented in the presence of neutralizing antibodies. In PCR experiments, a 
      distinct product was amplified with cDNA derived from cells treated with 0.1 ng 
      and 1 ng ml-1 TGF-beta 1, but not in control cultures. We conclude that TGF-beta 
      1 may play a role in differentiating TM cells towards a myofibroblast-like cell 
      type by modulating the expression of alpha-sm-actin.
FAU - Tamm, E R
AU  - Tamm ER
AD  - Department of Anatomy, University of Erlangen-Nurnberg, Germany.
FAU - Siegner, A
AU  - Siegner A
FAU - Baur, A
AU  - Baur A
FAU - Lutjen-Drecoll, E
AU  - Lutjen-Drecoll E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
RN  - 0 (Actins)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Actins/*biosynthesis
MH  - Aged
MH  - Aged, 80 and over
MH  - Animals
MH  - Humans
MH  - Immunohistochemistry
MH  - Macaca fascicularis
MH  - Muscle, Smooth/*metabolism
MH  - Polymerase Chain Reaction
MH  - Stimulation, Chemical
MH  - Trabecular Meshwork/drug effects/*metabolism
MH  - Transforming Growth Factor beta/*pharmacology
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - S0014-4835(96)90044-5 [pii]
AID - 10.1006/exer.1996.0044 [doi]
PST - ppublish
SO  - Exp Eye Res. 1996 Apr;62(4):389-97. doi: 10.1006/exer.1996.0044.