PMID- 8796421
OWN - NLM
STAT- MEDLINE
DCOM- 19961024
LR  - 20190905
IS  - 0168-1605 (Print)
IS  - 0168-1605 (Linking)
VI  - 29
IP  - 2-3
DP  - 1996 Apr
TI  - Enumeration of xerophilic yeasts in the presence of xerophilic moulds: a 
      collaborative study.
PG  - 185-92
AB  - A collaborative study was undertaken to compare the performance of Dichloran 18% 
      Glycerol agar (DG18) with three other media widely used in food mycology, for the 
      enumeration of xerophilic yeasts in the presence of xerophilic moulds. 
      Oxytetracycline Glucose Yeast extract agar, (OGY), Dichloran Rose Bengal 
      Chloramphenicol agar (DRBC) and Malt extract Yeast extract 50% Glucose agar 
      (MY50G) were evaluated. Three reference samples (A, B, C) were prepared using 
      skimmed milk powder inoculated with mixed lyophilized cultures of selected 
      xerophilic yeasts and moulds, at levels around 10(4) to 10(5) CFU/g. Yeast 
      species used were Candida glucosophila, C. versatilis, Zygosaccharomyces bailii 
      and Z. rouxii, together with Eurotium spp. and some other moulds. Collaborators 
      were asked to examine each sample twice, by dilution plating on the four media. 
      Colonies were counted after 3, 5 and 7 days incubation at 25 degrees C. Fourteen 
      participants from seven laboratories in six countries collaborated in this study. 
      The ability of collaborators to detect and count yeasts after 5 and 7 days on 
      DG18, DRBC and OGY varied depending on the sample. Few participants were able to 
      count yeasts even after 7 days on MY50G. The overall results demonstrated that 
      DG18 incubated 5 days at 25 degrees C was superior to the other three media 
      tested for the enumeration of xerophilic yeasts in the presence of xerophilic 
      moulds.
FAU - Braendlin, N
AU  - Braendlin N
AD  - Nestec Ltd., Research Centre Nestle, Lausanne, Switzerland.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Int J Food Microbiol
JT  - International journal of food microbiology
JID - 8412849
RN  - 0 (Culture Media)
SB  - IM
MH  - Candida/growth & development
MH  - Colony Count, Microbial
MH  - Culture Media/*chemistry
MH  - Mitosporic Fungi/*growth & development
MH  - Penicillium/growth & development
MH  - Reproducibility of Results
MH  - Time Factors
MH  - Yeasts/*growth & development
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - 0168-1605(95)00045-3 [pii]
AID - 10.1016/0168-1605(95)00045-3 [doi]
PST - ppublish
SO  - Int J Food Microbiol. 1996 Apr;29(2-3):185-92. doi: 10.1016/0168-1605(95)00045-3.