PMID- 8798918
OWN - NLM
STAT- MEDLINE
DCOM- 19970205
LR  - 20190914
IS  - 1572-6495 (Print)
IS  - 1572-6495 (Linking)
VI  - 681
IP  - 1
DP  - 1996 May 31
TI  - Separation of common nucleotides, mono-, di- and triphosphates, by capillary 
      electrophoresis.
PG  - 99-105
AB  - A capillary electrophoretic procedure for the separation of eleven nucleotides, 
      5'-mono-, di- and triphosphates of adenosine, guanosine, cytidine and uridine, 
      has been developed. All eleven analytes can be separated in a fused-silica 
      capillary (63 cm to the detector, I.D. 75 microns) at 20 kV in a 0.02 mol 1(-1) 
      phosphate-borate buffer (pH 8.0-9.0) with a separation factor > or = 1. The 
      values of the Offord parameter calculated for individual nucleotides predict that 
      monophosphates will migrate faster than triphosphates, and in turn triphosphates 
      will precede diphosphates. By analogy, faster electroosmotic mobility (lower 
      electromigration) of purine nucleotides (AP, GP) can be explained by a more 
      voluminous structure of purine derivatives (two aromatic rings as compared to 
      pyrimidines). Generally speaking, all compounds separated follow the Offord 
      equation assuming that the triphosphate derivatives are ionized to the third 
      degree forming HL(3-) anions. This assumption is in agreement with the current 
      knowledge about protolytic equilibria of polyphosphates. The only exception to 
      this rule is faster migration of guanosine-5'-triphosphate (GTP) preceding 
      uridine-5'-monophosphate (UMP) which is ascribed in part to the larger molecule 
      of GTP and the two additional OH-groups bound to the pyrimidine ring of UMP.
FAU - Uhrova, M
AU  - Uhrova M
AD  - Institute of Chemical Technology, Prague, Czech Republic.
FAU - Deyl, Z
AU  - Deyl Z
FAU - Suchanek, M
AU  - Suchanek M
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Chromatogr B Biomed Appl
JT  - Journal of chromatography. B, Biomedical applications
JID - 9421796
RN  - 0 (Nucleotides)
RN  - 0 (Phosphates)
SB  - IM
MH  - Electrophoresis, Capillary/*methods
MH  - Hydrogen-Ion Concentration
MH  - Nucleotides/*analysis/chemistry
MH  - Phosphates/*analysis/chemistry
MH  - Reproducibility of Results
EDAT- 1996/05/31 00:00
MHDA- 1996/05/31 00:01
CRDT- 1996/05/31 00:00
PHST- 1996/05/31 00:00 [pubmed]
PHST- 1996/05/31 00:01 [medline]
PHST- 1996/05/31 00:00 [entrez]
AID - 0378434795005358 [pii]
AID - 10.1016/0378-4347(95)00535-8 [doi]
PST - ppublish
SO  - J Chromatogr B Biomed Appl. 1996 May 31;681(1):99-105. doi: 
      10.1016/0378-4347(95)00535-8.